Today i gave mrs. weissman my new graphs, and i gave mrs. weissman my data analysis, so she could give me pointers. i know most of it is wrong, but i felt like i had to write something over break.
this is all of my lab report i have so far:
Lab report:
Experimental question: “How does different levels smoke affect Drosophila melanogaster development?”
Hypothesis: “ if Drosophila melanogaster are subjected to varying levels of smoke, then the Drosophila melanogaster’s development will be affected.”
Materials:
-1 Graduated cylinder
- 1 pair of goggles
- Painters tape
- 4 73 mL plastic vials
- 1 black sharpie
- 1 plastic spoon
- 1 sheet of paper towel
- 1 1 oz bottle of blue food coloring
- 4 foam plugs
- 6 tea candles (optional: 6 more if the others run out)
- 1 metal box
- 1 ice pick
- 1 microscope
- 135 cc syringe
- 1 paintbrush
- 1 73 mL vial filled with adult fruit flies (ordered from Connecticut Valley Biological Supply)
- 1 magnifying glass
- 1 medium sized box that with not melt
- 1 lighter
- 1 petrie dish
- 1 microscope
- 1 box of matches
- 1 petrir dish lid
- 1 box of Fly Nap
- metal scooper?
Procedure:
1. Take the ice pick, and poke a hole big enough for the syringe tip to go through. Put one on the top and on the side of the metal box.
2. Put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. Repeat steps 1-4 three times.
6. Take the same graduated cylinder and fill it with 14 mL of water.
7. Take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the liquid a very dark blue.
9. Pour the blue mixture into the same vial with the fruit fly media.
10. Repeat steps 6-8 three times, pouring the mixture in the other vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
12. Take the painter tape, and put a strip of tape on the side of each vial.
13. Label one “control”, one “small amount of smoke”, one “medium amount of smoke”, one “large amount of smoke”.
14. Wait for the fruit flies to arrive in the mail, and when they do wait for many larva to hatch.
15. Once there are enough larva, get out Fly Nap
16. Open the box and take out the 4 black q-tips inside.
17. Unscrew the cap of the bottle of fly nap and take it off.
18. Take one of the black q-tips and dip it in the Fly Nap, and then pull it out so it is covered in Fly Nap.
19. put the same q-tip into the vial of fruit flys, pushing it donw the side of the vial past the foam plug so no flies escape.
20. Wait 4 minutes for the flies to fall asleep.
21. Once the flies are asleep, take out the foam plug, and nock most of the sleping flies into a petrie dish.
22. Using a metal scooper, scoop the fly larva (along with the medium it’s in) out.
23. Place the fruit larva in one of the empty vials.
24. Repeat steps 15-23 three times.
25. Take the metal box, and take the lid off of it.
26. Light a match and light all the candles in the metal box.
27. Blow them out fast, and slam the lid on top.
28. Wait 10 minutes for the smoke and the air inside to mingle.
29. Take the tape off the top hole of the box and quickly stick the syringe in.
30. Pull out 11 cc of smoke using the syringe.
31. Take the syringe and put the tape back on before any smoke escapes.
32. stick the tip in the side of one of the vials, not taking the plug out
33. Push in the 11 cc of smoke, and pull the tip back out.
34. Repeat steps 25-33 twice, except instead of drawing out 11 ccs draw out 22 cc one time and 33 cc the next.
35. Leave one vial without any smoke. It is the control.
36. Every other day repeat 25-35.
37. Once the flies have hatched out of the puparium and are adults, use fly nap and repeat steps 15- 20.
38. Take out the foam plug and nock out all the flies from the vial onto the petrie dish.
39. Count the number of flies on the dish.
40. Record the number of flies.
41. Nock the flies back into the vial.
42. Place the foam plug back in.
43. Repeat 37-42 with the “small amount of smoke”, “medium amount of smoke”, and “large amount of smoke”.
44. Once done counting the flies fomr each vial, repeat steps 25-35.
45. After about a week there will be New larva in the vials.
46. Repeat steps 3-23, using red food coloring instead of blue.
47. Continue what you do everyday (steps 37-44), except now do it wit the vials wit the blue fruit fly media and vials wit the red fruit fly media.
Independent variable: Different levels of candle smoke
Dependent variable: Fruit fly development
Observations:
date Control Small amount of smoke Medium amount of smoke Large amount of smoke
2/4/10 They seems to be not a lot of larva in the control They are moving around and eating They are moving around and eating They are moving around and eating
2/9/10 Some larva have hatched, and some have gone into the puparium and are undergoing metemorphis. From what I can see 7 new adlut flies have hatched.
Tgere are many in the puparium, but they are to hard ot count.
There are still some larva left. 6 flies have under gone matamorphis and have emerged as an adult fly. There are still some in the puparium though.
I saw 1 larva moving around, so I assume there is more. So far 1 fly has emerged, the rest are in the puparium. A few of the pupariums have darkens, so I know the adult fly will emerge soon. 1 fruit fly has emerged, and the rest are in the puparium. Once again, a few of the pupariums have darkened so I know flys will emerge soon.
2/16/10 The flies are hoping around a lot!!! The flies are not hoping around are as the control. there are less hatched flies The flies are not as active as the control. there are less hatched flies The flies are hoping not as much as control, and there is almost just as much hatched flies in the vial.
2/17/10 There aren’t many puparium, and the oens that are there are darker so that means they’re about to emerge. The flys are flying around, there are some puparium but they are lgiht The flys are flying around, there are some puparium but they are lgiht The flys are flying around, there are some puparium but they are lgiht
Date Observations
2/4/10 They seems to be not a lot of larva in the control
They are moving around and eating
Many are moving around and eating
Many are moving around and eating
2/9/10 Some larva have hatched, and some have gone into the puparium and are undergoing metemorphis. From what I can see 7 new adlut flies have hatched.
Tgere are many in the puparium, but they are to hard ot count.
There are still some larva left.
6 flies have under gone matamorphis and have emerged as an adult fly. There are still some in the puparium though.
I saw 1 larva moving around, so I assume there is more.
So far 1 fly has emerged, the rest are in the puparium. A few of the pupariums have darkens, so I know the adult fly will emerge soon.
1 fruit fly has emerged, and the rest are in the puparium. Once again, a few of the pupariums have darkened so I know flys will emerge soon.
2/16/10 The flies are hoping around a lot!!!
The flies are not hoping around are as the control. there are less hatched flies
The flies are not as active as the control. there are less hatched flies
The flies are hoping not as much as control, and there is almost just as much hatched flies in the vial.
2/17/10 There aren’t many puparium, and the oens that are there are darker so that means they’re about to emerge.
The flys are flying around, there are some puparium but they are lgiht
The flys are flying around, there are some puparium but they are lgiht
The flys are flying around, there are some puparium but they are lgiht
Date Amount of adults fruit flies when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of adults fruit flies when subjected to 22 ml of smoke Amount of adults fruit flies when subjected to 33 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 11 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 22 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 33 ml of smoke
2/22/10 55 10 10 12 6 1 0 0
2/24/10 65 27 8 8 8 9 0 0
3/1/10 20 100 20 61 12 36 2 12
3/3/10 19 101 22 25 3 27 0 12
3/5/10 16 111 6 40 1 4 2 9
3/8/10 12 100 6 41 21 1 4 3
3/10/10 12 105 4 28 71 17 2 1
DATA TABLE 1:
The average amount of adult frit flies when subjected to different amounts of smoke over time
DATA TABLE 2:
The Average Amount of Fruit Fly Pupa When Subjected to Different Amounts of Smoke Over Time
Date Amount of fruit fly pupa when subjected to 0 ml of smoke Amount of fruit fly pupa when subjected to 11 ml of smoke Amount of fruit fly pupa when subjected to 22 ml of smoke Amount of fruit fly pupa when subjected to 33 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 0 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 11 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 22 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 33 ml of smoke
3/2/10 76 120 7 13 25 53 4 5
3/3/10 90 138 11 22 30 50 4 6
3/4/10 89 140 8 21 42 41 4 5
3/5/10 96 152 8 18 55 88 4 4
3/8/10 109 155 10 23 85 109 3 6
3/9/10 110 188 9 20 83 107 2 6
3/10/10 95 140 9 25 89 131 4 6
The Average Amount of Fruit Fly Pupa When Subjected to Different Amounts of Smoke
Amount of smoke Average of fruit fliy pupa
0 ml of smoke 95
11 ml of smoke 148
22 ml of smoke 9
33 ml of smoke 20
0 ml of smoke F1 generation 58
11 ml of smoke F1 generation 83
22 ml of smoke F1 generation 4
33 ml of smoke F1 generation 6
The average amount of adult frit flies when subjected to different amounts of smoke
Amount of Smoke Average of adult flies
0 ml of smoke 28
11 ml of smoke 79
22 ml of smoke 11
33 ml of smoke 31
0 ml of smoke F1 generation 17
11 ml of smoke F1 generation 13
22 ml of smoke F1 generation 2
33 ml of smoke F1 generation 5
Graph 1:
Graph 2:
Data analysis:
This experiment focused on the affect of smoke on the development of fruit flies, and if there is an effect, determining how extreme it is. Acknowledging the fact that different amounts of smoke could affect the different rates of development of fruit flies, different amounts of smoke were used in this experiment to determine the nature of their relationship. Two kinds of data were taken: the number of live adult fruit flies on a certain day, and the number of fruit fly pupa on a certain day. The results were recorded as quantitative data, using fly nap as an anesthetizer to quite the flies so they could be counted, and a magnifying glass to clearly see the number of fly pupa.
The number of adult fruit flies when subjected to different amounts of smoke over time was recorded in data table 1, and the average per vial of those adult flies was recorded in data table number 3. There were many noticeable trends in these data tables. For one, the number of adult fruit flies was a great deal higher then the number of fruit flies in any other container, including the control. In both the parent generation and the f1 generation, the highest number of live adult fruit flies was in the vial subjected to small amount of smoke. Another notable trend was the fact that number of adult fruit flies that were subjected to the medium amount of smoke was lower than any other vial, including the number of fruit flies that were subjected to the highest amount of smoke. Once again, this trend occurs both in the parent generation and the f1 generation. The highest number of live adult fruit flies in the medium amount of smoke vial was 22, which is very low compared to all the other vials. The vial that held fruit flies that were subjected to the greatest amount of smoke was supposed to have the least amount of flies in it actually had more flies then the vial that had the medium amount of smoke in it, except for one day when both vials had 8 flies. Looking at data table 3 you can see that over all the vial subjected to the least amount of fruit flies had the most live adult flies, the vial subjected to no smoke had the sound largest number of living adult flies, the vial subjected to the largest amount of smoke had the third greatest amount of live adult fruit flies, and the vial that was subjected to the medium amount of smoke had the least amount of live adult fruit flies.
The number of fruit fly pupa each day was recorded in data table 2, and the average of those pupa was recorded in data table 4. A noticeable trend in both of these data tables is the fact that the highest number of pupa was in the vials that were subjected to the least amount of smoke. This trend resembles that of data tables 1 and 3. Another pattern in these data tables are the facts that the number pupa in the vials subjected to medium amount of smoke was very low, and the number of pupa in the vial subjected to a largest amount of smoke was higher. Once again, this trait resembles that of data table 1 and 3.
In conclusion, all 4 of these data tables resemble each other’s patterns and trends. The largest amount of live fruit flies and the largest amount of pupa for both generations occurred in the vial that was subjected to the smallest amount of smoke. It is important to remember that these numbers were higher then those in the vials with no smoke, which was not something that was expected to, happen. The other main trend was the fact that lowest number of live adult fruit flies and the lowest number of pupa occurred in the vials that were subjected to the medium amount of smoke. Another important thing to remember that these numbers were lower then those in the vials subjected to largest amount of smoke, which is another trend that was not predicted to occur.
Conclusion:
Monday, March 29, 2010
Thursday, March 11, 2010
Thursday March 11th, 2010
Today I cleaned up my experiment. It was almost sad, but i as happy to see those flies go. They were DISGUSTING! I put the flies in the freezer, so I guess they'll freeze. I also went to see Mrs. Weissman to ask how I should write my data analysis. I think i got a basic idea, but i'll see what she says to us in class. Today I wrote my intro to my analysis i think. this is what I wrote:
Data analysis:
This experiment focused on the affect of smoke on the development of fruit flies, and if there is an affect, determine how extreme it is. Acknowledging the fact that different amounts of smoke could affect the different rates of development of fruit flies, different amounts of smoke were used in this experiment to determine the nature of their relationship. Two kinds of data were taken: the number of live adult fire flies on a certain day, and the number of fruit fly pupa on a certain day. The results were recorded as quantitative data, using fly nap as a anestisiser to quite the flies so they could be counted, and a magnifying glass to clearly see the number of pupa.
That is what i've written so far. I hope it's right.
Data analysis:
This experiment focused on the affect of smoke on the development of fruit flies, and if there is an affect, determine how extreme it is. Acknowledging the fact that different amounts of smoke could affect the different rates of development of fruit flies, different amounts of smoke were used in this experiment to determine the nature of their relationship. Two kinds of data were taken: the number of live adult fire flies on a certain day, and the number of fruit fly pupa on a certain day. The results were recorded as quantitative data, using fly nap as a anestisiser to quite the flies so they could be counted, and a magnifying glass to clearly see the number of pupa.
That is what i've written so far. I hope it's right.
Wednesday, March 10, 2010
Wensday march 10th, 2010
Today i finished my experiment!!!!!!!!!! I'm so happy!!!!! those flies were really disgusting. tomorrow I'm going to c ms. Weissman about how to write a data analysis and a conclusion. also, i need to talk to her about what caused my data, because i have no clue still. i have lots to talk to her about. Today i also took lots of photos on my laptop of my experimental design to include in my lab report. I'm going to ask Nadia tomorrow how to label them on the computer.
Tuesday, March 9, 2010
Tuesday, March 9th, 2010
Today I made my graphs. surprisingly, it was not hard to do! I'm going to the graphing class anyway at CWP just to be safe, but I've successfully made a graph!!! actually I've made 2 graphs! they are very complicated graphs, but everything about them is right i think!! the only problem is they are very very hard to read, so I'm really going to have to explain it when I present. I can't post graphs though, so I'll email them to myself ot back them up!
I think I will ask ms. Weissman about how to write my data analysis tomorrow.
I think I will ask ms. Weissman about how to write my data analysis tomorrow.
Monday, March 8, 2010
Monday, March 8th, 2010
Today us my second to last day using fly nap to count the adult flies. I'm very excited. I turned my data table in today, so i got those points, which is good. I'm very worried about one thing though. I have no clue why there are so many bugs in "small amount of smoke". And that goes for both the f1 generation and the f2 generation. smoke can't be actually good for them, can it? I'm very confused ,and I'm worried about what I'll say in my conclusion. I'm also worried about writing my data analysis. i know it's supposed to be long, but i do not have a lot data, so I'm not really sure what i write about. Today i got more data, which is good. I counted the adult flies and he pupa. this is what i got.
PUPA:(in the order of: control, small, medium, large, control f2, small f2, medium f2, large f2)
3/8/10 109 155 10 23 85 109 3 6
ADULTS: (in the order of: control, small, medium, large, control f2, small f2, medium f2, large f2)
3/8/10 12 100 6 41 21 1 4 3
PUPA:(in the order of: control, small, medium, large, control f2, small f2, medium f2, large f2)
3/8/10 109 155 10 23 85 109 3 6
ADULTS: (in the order of: control, small, medium, large, control f2, small f2, medium f2, large f2)
3/8/10 12 100 6 41 21 1 4 3
Saturday, March 6, 2010
Saturday March 6th
Yesterday I went and talked to ms. Weissman, and decided i need to make new data tables. Now i'm going ot have 2 different data tables, one for number of adutls, and one for number of pupa. this is what i ended up with:
Date Amount of adults fruit flies when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of adults fruit flies when subjected to 22 ml of smoke Amount of adults fruit flies when subjected to 33 ml of smoke
2/22/10 55 10 10 12
2/24/10 65 27 8 8
3/1/10 20 100 20 61
3/3/10 19 101 22 25
3/5/10 16 111 6 40
Date Amount of fruit fly pupa when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of fruit fly pupa when subjected to 22 ml of smoke Amount of fruit fly pupa when subjected to 33 ml of smoke
3/2/10 76 120 7 13
3/3/10 90 138 11 22
3/4/10 89 140 8 21
3/5/10 96 152 8 18
Date Amount of fruit fly pupa of the F2 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 11 ml of smoke Amount of fruit fly pupa of the F2 generation when subjected to 22 ml of smoke Amount of fruit fly pupa of the F2 generation when subjected to 33 ml of smoke
3/2/10 25 53 4 5
3/3/10 30 50 4 6
3/4/10 42 41 4 5
3/5/10 55 88 4 4
Date Amount of adults fruit flies of the F2 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 11 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 22 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 33 ml of smoke
2/22/10 6 1 0 0
2/24/10 8 9 0 0
3/1/10 12 36 2 12
3/3/10 3 27 0 12
3/5/10 1 4 2 9
it's hard t format in this blog. my data makes no sense to me what's so ever, and I need ot go see ms. weissman when i'm done taking it. I'm very worried that i won't be able to explain it in my conclusion, because i have ablutly no clue why the small amount of smoke is so much higher the the control. it can't just be because of the excess amount of fly nap, because that same trand is in the f2 gernation as well. I hope i can figure out what happened.
Date Amount of adults fruit flies when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of adults fruit flies when subjected to 22 ml of smoke Amount of adults fruit flies when subjected to 33 ml of smoke
2/22/10 55 10 10 12
2/24/10 65 27 8 8
3/1/10 20 100 20 61
3/3/10 19 101 22 25
3/5/10 16 111 6 40
Date Amount of fruit fly pupa when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of fruit fly pupa when subjected to 22 ml of smoke Amount of fruit fly pupa when subjected to 33 ml of smoke
3/2/10 76 120 7 13
3/3/10 90 138 11 22
3/4/10 89 140 8 21
3/5/10 96 152 8 18
Date Amount of fruit fly pupa of the F2 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 11 ml of smoke Amount of fruit fly pupa of the F2 generation when subjected to 22 ml of smoke Amount of fruit fly pupa of the F2 generation when subjected to 33 ml of smoke
3/2/10 25 53 4 5
3/3/10 30 50 4 6
3/4/10 42 41 4 5
3/5/10 55 88 4 4
Date Amount of adults fruit flies of the F2 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 11 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 22 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 33 ml of smoke
2/22/10 6 1 0 0
2/24/10 8 9 0 0
3/1/10 12 36 2 12
3/3/10 3 27 0 12
3/5/10 1 4 2 9
it's hard t format in this blog. my data makes no sense to me what's so ever, and I need ot go see ms. weissman when i'm done taking it. I'm very worried that i won't be able to explain it in my conclusion, because i have ablutly no clue why the small amount of smoke is so much higher the the control. it can't just be because of the excess amount of fly nap, because that same trand is in the f2 gernation as well. I hope i can figure out what happened.
Thursday, March 4, 2010
Thursday, march 4th, 2010
Today I did not count my fruit flies, because i don't want to kill them by anesticising them everyday. I'm kind of worried about my lack of data (4 days counting flies, 2 counting pupa) but I hope I will have enough to graph by Wednesday. this what i got today:
3/4/10 Control Pupa: 89 Control F2 Pupa: 42
Small Pupa: 140 Small f2 Pupa: 41
Medium Pupa: 8 Medium F2 Pupa:4
Large Pupa: 21 Large F2 Pupa: 5
Before my data was all messed up because i wasn't able to add smoke to the fruit flies due to the snow days, but that i've been doing it for a few days finally i think my data is getting back on track, or atleast the pupa data is. yesterday there were 30 pupa in the F2 control vial and now there is 42. the flies are developing fast in the control, and not as fast in any of the others. Maybe i will end up with the results i predicited! Only time will tell.
3/4/10 Control Pupa: 89 Control F2 Pupa: 42
Small Pupa: 140 Small f2 Pupa: 41
Medium Pupa: 8 Medium F2 Pupa:4
Large Pupa: 21 Large F2 Pupa: 5
Before my data was all messed up because i wasn't able to add smoke to the fruit flies due to the snow days, but that i've been doing it for a few days finally i think my data is getting back on track, or atleast the pupa data is. yesterday there were 30 pupa in the F2 control vial and now there is 42. the flies are developing fast in the control, and not as fast in any of the others. Maybe i will end up with the results i predicited! Only time will tell.
Wednesday, March 3, 2010
3/310
Today I used fl nap and counted my flies. I hope the fly nap is not killing them. I am really worried, Dr. Wright said I could be killing them. I hope collecting the data everyother day gets rid of the that problem. I wish I didn't have to do it, because I'm loosing a chance to get as mcuh data as i can. But i guess killing all the flies would not give me any data either. I have not started my lab report, or my data analisis. I might put everything in a document just to get a start. this is data i got today:
3/3/10 Control 19 adults
Pupa: 90 Control F2 3 adults
Pupa: 30
Small 101 adults
Pupa: 138 Small f2 27 adults
Pupa: 50
Medium 22 adults
Pupa: 11 Medium F2 0 adults
Pupa: 4
Large 25 adults
Pupa: 22 Large F2 12 adults
Pupa: 6
3/3/10 Control 19 adults
Pupa: 90 Control F2 3 adults
Pupa: 30
Small 101 adults
Pupa: 138 Small f2 27 adults
Pupa: 50
Medium 22 adults
Pupa: 11 Medium F2 0 adults
Pupa: 4
Large 25 adults
Pupa: 22 Large F2 12 adults
Pupa: 6
Tuesday, March 2, 2010
March 2, 2010
Today I decided I would not anestise why flies everyday. because it, turns it kills them. Darn. I edited my procedure though, and this is what i came up with:
it's not very good, and the steps will probably have changed by the time I write my pab report, but it is as good as i can get it.
Procedure:
1. Take the ice pick, and poke a hole big enough for the syringe tip to go through. Put one on the top and on the side of the metal box.
2. Put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. Repeat steps 1-4 three times.
6. Take the same graduated cylinder and fill it with 14 mL of water.
7. Take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the liquid a very dark blue.
9. Pour the blue mixture into the same vial with the fruit fly media.
10. Repeat steps 6-8 three times, pouring the mixture in the other vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
12. Take the painter tape, and put a strip of tape on the side of each vial.
13. Label one “control”, one “small amount of smoke”, one “medium amount of smoke”, one “large amount of smoke”.
14. Wait for the fruit flies to arrive in the mail, and when they do wait for many larva to hatch.
15. Once there are enough larva, get out Fly Nap
16. Open the box and take out the 4 black q-tips inside.
17. Unscrew the cap of the bottle of fly nap and take it off.
18. Take one of the black q-tips and dip it in the Fly Nap, and then pull it out so it is covered in Fly Nap.
19. put the same q-tip into the vial of fruit flys, pushing it donw the side of the vial past the foam plug so no flies escape.
20. Wait 4 minutes for the flies to fall asleep.
21. Once the flies are asleep, take out the foam plug, and nock most of the sleping flies into a petrie dish.
22. Using a metal scooper, scoop the fly larva (along with the medium it’s in) out.
23. Place the fruit larva in one of the empty vials.
24. Repeat steps 15-23 three times.
25. Take the metal box, and take the lid off of it.
26. Light a match and light all the candles in the metal box.
27. Blow them out fast, and slam the lid on top.
28. Wait 10 minutes for the smoke and the air inside to mingle.
29. Take the tape off the top hole of the box and quickly stick the syringe in.
30. Pull out 11 cc of smoke using the syringe.
31. Take the syringe and put the tape back on before any smoke escapes.
32. stick the tip in the side of one of the vials, not taking the plug out
33. Push in the 11 cc of smoke, and pull the tip back out.
34. Repeat steps 25-33 twice, except instead of drawing out 11 ccs draw out 22 cc one time and 33 cc the next.
35. Leave one vial without any smoke. It is the control.
36. Every other day repeat 25-35.
37. Once the flies have hatched out of the puparium and are adults, use fly nap and repeat steps 15- 20.
38. Take out the foam plug and nock out all the flies from the vial onto the petrie dish.
39. Count the number of flies on the dish.
40. Record the number of flies.
41. Nock the flies back into the vial.
42. Place the foam plug back in.
43. Repeat 37-42 with the “small amount of smoke”, “medium amount of smoke”, and “large amount of smoke”.
44. Once done counting the flies fomr each vial, repeat steps 25-35.
45. After about a week there will be New larva in the vials.
46. Repeat steps 3-23, using red food coloring instead of blue.
47. Continue what you do everyday (steps 37-44), except now do it wit the vials wit the blue fruit fly media and vials wit the red fruit fly media.
it's not very good, and the steps will probably have changed by the time I write my pab report, but it is as good as i can get it.
Procedure:
1. Take the ice pick, and poke a hole big enough for the syringe tip to go through. Put one on the top and on the side of the metal box.
2. Put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. Repeat steps 1-4 three times.
6. Take the same graduated cylinder and fill it with 14 mL of water.
7. Take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the liquid a very dark blue.
9. Pour the blue mixture into the same vial with the fruit fly media.
10. Repeat steps 6-8 three times, pouring the mixture in the other vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
12. Take the painter tape, and put a strip of tape on the side of each vial.
13. Label one “control”, one “small amount of smoke”, one “medium amount of smoke”, one “large amount of smoke”.
14. Wait for the fruit flies to arrive in the mail, and when they do wait for many larva to hatch.
15. Once there are enough larva, get out Fly Nap
16. Open the box and take out the 4 black q-tips inside.
17. Unscrew the cap of the bottle of fly nap and take it off.
18. Take one of the black q-tips and dip it in the Fly Nap, and then pull it out so it is covered in Fly Nap.
19. put the same q-tip into the vial of fruit flys, pushing it donw the side of the vial past the foam plug so no flies escape.
20. Wait 4 minutes for the flies to fall asleep.
21. Once the flies are asleep, take out the foam plug, and nock most of the sleping flies into a petrie dish.
22. Using a metal scooper, scoop the fly larva (along with the medium it’s in) out.
23. Place the fruit larva in one of the empty vials.
24. Repeat steps 15-23 three times.
25. Take the metal box, and take the lid off of it.
26. Light a match and light all the candles in the metal box.
27. Blow them out fast, and slam the lid on top.
28. Wait 10 minutes for the smoke and the air inside to mingle.
29. Take the tape off the top hole of the box and quickly stick the syringe in.
30. Pull out 11 cc of smoke using the syringe.
31. Take the syringe and put the tape back on before any smoke escapes.
32. stick the tip in the side of one of the vials, not taking the plug out
33. Push in the 11 cc of smoke, and pull the tip back out.
34. Repeat steps 25-33 twice, except instead of drawing out 11 ccs draw out 22 cc one time and 33 cc the next.
35. Leave one vial without any smoke. It is the control.
36. Every other day repeat 25-35.
37. Once the flies have hatched out of the puparium and are adults, use fly nap and repeat steps 15- 20.
38. Take out the foam plug and nock out all the flies from the vial onto the petrie dish.
39. Count the number of flies on the dish.
40. Record the number of flies.
41. Nock the flies back into the vial.
42. Place the foam plug back in.
43. Repeat 37-42 with the “small amount of smoke”, “medium amount of smoke”, and “large amount of smoke”.
44. Once done counting the flies fomr each vial, repeat steps 25-35.
45. After about a week there will be New larva in the vials.
46. Repeat steps 3-23, using red food coloring instead of blue.
47. Continue what you do everyday (steps 37-44), except now do it wit the vials wit the blue fruit fly media and vials wit the red fruit fly media.
Wednesday, February 24, 2010
Febuary 24th 2010
I was sick yesterday so i couldn't collect data. I have deicided that i will count the friut flies, and that will be data. this is what i have collected os far:
2/22/10 control 55 adults Control F2 6
small 10 adults Small F2 1
Medium 10 adults Medium F2 none
Large 12 adults Large F2 none
2/24/10 Control Control F2 8 adults
Small 27 adults Small f2
Medium 8 adults Medium F2 none
Large 8 adults Large F2 none
THe control flies wouldn't fall alsleep and so wouldn't hte small F2, so i will do that at hte end of the day.
I submited my research paper.
2/22/10 control 55 adults Control F2 6
small 10 adults Small F2 1
Medium 10 adults Medium F2 none
Large 12 adults Large F2 none
2/24/10 Control Control F2 8 adults
Small 27 adults Small f2
Medium 8 adults Medium F2 none
Large 8 adults Large F2 none
THe control flies wouldn't fall alsleep and so wouldn't hte small F2, so i will do that at hte end of the day.
I submited my research paper.
Thursday, February 18, 2010
Thursday, febuary 18th, 2010
Today I set up the vials for the F2 generation of my fruit flies. I did the same egsact thing I did with the theee F1 Generation, but instead I used red food coloring. I need to work on my research paper, and will see ms. weissman tomorrow. Today i did not get to take data. I feel like my experiment in this genreration is ruined due to the6 day weekend, but i an optoistic about this next one. I pla to transfer the bug larva monday, so i'll have a week to observe.
This is my research paper:
Research Paper:
The topic that is being studied is the affect of air pollution on insect development. It is a very important issue that needs to be addressed. If air pollution has an affect on insects and their development, then it could affect humans, and also potentially the entire global ecosystem! This could happen easily if humans are not careful about air pollution. Though insects do not seem important compared to the rest of the world, they are the base of the food chain, and play a very important role in the ecosystem. This is because in an environment everything affects everything. If air pollution affects insect development, then animals that eat insects (such as frogs) could lose their food, and start to die out. Then animals that eat frogs (such snakes, lizards, birds, and small animals like hedge hogs) could lose food and start to die out, and so on. Everything affects everything in the food chain. A small problem could work its way up and wreck an entire ecosystem. If air pollution is causing a major problem with insects, then there could end up being a major problem with humans and the worldwide environment. That is why it is so important that the affect of air pollution on insects be studied.
The way to study this topic is by conducting an experiment that studies the affect of different levels of smoke on Drosophila melanogaster development. The independent variable will be different levels of smoke, which is a collection of airborne solid and liquid particles and gases that are emitted when combustion takes place. The make-up of smoke depends on what kind of a fuel is burning (“Smoke”, 2010). Smoke is a very good representation for air pollution, because it makes up many different kinds of air pollution, in particular black carbon air pollution, which is smoke from factories and other burning fuel (“Indoor and Outdoor Air Pollution”, 2010). The dependent variable will be the development of Drosophila melanogaster, otherwise known as fruit flies. Fruit flies are very common insects so they are a good representation of insects in this experiment. Fruit flies are small insects that have red eyes, a tan front portion of their body, and black rings across their back abdomen. They lay many eggs, breed quickly, are easy to take care of, and their life cycle is around 30 days when at 29 °C (84 °F) (“Drosophila melanogaster” 2010). Together these two variables would represent the affect of air pollution of insect development.
The two variables that are being studied are smoke, and fruit fly development. The dependent variable is fruit fly development. The scientific name for fruit fly is Drosophila melanogaster, and they are incredibly common insects. A fruit fly has four stages in life: egg, larva, pupa, and adult (“Life Cycle of a Fruit Fly”, 2010). Their nickname fruit fly originates from the fact they are often found around fruit that has been left alone, or is going bad. They do this because when a female fruit fly is ready to lay eggs, she lays them in moist fermenting food, or other organic decomposing materials. This is because once a larva (which is a baby insect) emerges from its egg; it will feed on the sugar from the material it was laid in, and also on the microorganisms that are decomposing that material (“Drosophila melanogaster” 2010). The development of a fruit fly is fairly simple. Once a fruit fly hatches, it goes through two molting periods, in which many parts are shed. Before and after these times of growth, a larva is called an instar, and there are three times when they are called that. After the third instar, the pauperism (which is the outer shell of the bug) becomes harder and darker than before. Within the pauperism is where the metamorphis takes place, which is when a larva turns into an adult. A person can tell when a fly is about to emerge from the puparium when it darkens. Once the metamorphis is completed, the new adult fly pushes it’s way out of the front end of the pauperism. Right after the adult fly emerges it is light with a long abdomen and folded wings, but a few hours later the fly turns darker, it spreads out its wings, and its abdomen becomes rounder. About 48 hours after a female fly emerges it can lay eggs, and the process begins again. A fly spends 8 days as an egg and in larval stages, then 6 days in the pupal stages. Its life span is several weeks and at normal room temperature (21 Co). A whole new population of fruit flies can be created in two weeks (“Life Cycle of a Fruit Fly”, 2010).
Varying levels of smoke is the independent variable. The definition of smoke is: “the gaseous products of burning materials especially of organic origin made visible by the presence of small particles of carbon” (“smoke”, 2010). To state that differently, it is the collection of airborne solid and liquid particles and gases. It occurs when a material undergoes combustion, and the nature of it depends on what is burning. Fires that have large amounts of oxygen around them burn at higher temperatures and they emit a smaller amount of smoke. Fires that burn with a lack of oxygen produce a much larger palette of compounds, and many of those compounds are toxic. Smoke can be visible or invisible to the naked eye, depending on the size of the particles. A good example of this is when you toast a piece of bread in a toaster. As the bread heats up, the products of combustion begin to get larger. The particles that are produced by the toast at first are invisible, but if the toast burns, the particles become visible. Smoke also can also be incredibly dangerous. In an average house fire for example, the smoke contains hundreds and hundreds of different chemicals and fumes. Usually the damage on the house is much greater from the smoke then from, the heat of the fire (“Smoke”, 2010).
The relationship between abiotic factors and biotic factors in the environment is very important. An abiotic factor is “Any of the nonliving factors that make up the abiotic environment in which living organisms occur.”(“abiotic factors”, 2004) To put it more simply, anything in an environment that is not living and didn’t come from something living is abiotic (for example, a nut is not abiotic because it comes from a tree). There are many examples of abiotic factors such as: temperature, weather, fire, gas, natural disasters (floods, earth quakes), elevation, UV rays, acid rain, humidity, air pollution, air pressure, the list goes on and on, and none of then come form something living. A biotic factor is “Any of the factors of an organism's environment that consist of other living organisms and together make up the biotic environment” (“biotic factors”, 2004) it is the opposite of an abiotic factor, because it is anything in an environment that is living and/or did come from something living (for example, a nut is biotic). Examples of biotic factors are: food (or lack of food), disease, predation, old age, injury, and so on. These two different kinds of factors are incredibly important in ecosystems.
In the experiment that is being conducted, smoke is the abiotic factor, and fruit fly development is the biotic factor. Just like in any environment, the abiotic factor affects the biotic factor. Some examples of this are when it is cold outside and a plant freezes and dies, or if there is not enough CO2 in the air and a plant dies because it cannot go through photosynthesis and produce glucose. Biotic factors are always affected by abiotic factors. In this experiment, the smoke affects the fruit fly development. This relationship is very important, because if smoke affects fruit flies it could end up affecting humans. That is because of the way the food chain works. First, stop being so specific and change the dependent variable of fruit flies to insects in general. Also, remember smoke is in many different kinds of air pollutions. Because of this, if smoke affects fruit flies and their development, then many different kinds of air pollution could affect insects and their development. If insects are affected and/or are developing differently due to air pollution, then their predators such as birds might lose a major source of food, because what they had been eating is no longer as common or has changed in a way that birds aren’t adapted to dealing with. Because of this lose of food birds and their survival rate could begin to diminish. Then animals that eat birds, like foxes, could lose their food source and their population could begin to diminish. Then the population of animals that eat foxes start to diminish, and its predator loses food, and so on and so on. It just works its way up the food chain, knocking everything out of balance. Eventually the entire global ecosystem could be trashed! So even though the change in insect development may seem like a minor problem, in the long run it can lead to a major issue.
This experiment is being studied for one reason. To figure out if air pollution has a major effect on ecosystems, and could eventually end up affecting the entire global eco-system. The independent variable is smoke, because smoke is a common component in air pollution, and it is a safe clean way to represent air pollution in an experiment. The dependent variable is fruit flies, because they are common insects that are easy to handle with large reproductive potential, and that is why they are good representatives for insects in general. If the smoke affects fruit fly development, then it can be assumed that air pollution can affect insect development. If insect development is compromised, then thousands of ecosystems could be destroyed! So, though it may not seem like it at first, the study of the affect of smoke on fruit fly development is incredibly important to our global ecosystem. That is why it is being studied.
References
“abiotic factor.” A Dictionary of Biology. 2004. Retrieved January 19, 2010 from E. (2004). abiotic factor. Retrieved January 19, 2010, from Encyclopedia.com database.
Air Pollution. (n.d.). Retrieved January 13, 2010, from Lawrence Berkeley National Laboratory website: http://www.lbl.gov/Education/ELSI/pollution-main.html
Atkinson, E. (2009, February 21). What is Smoke? Retrieved from http://chestofbooks.com/reference/The-New-Student-s-Reference-Work-Vol5/What-Is-Smoke.html
biotic factors. (2004). Retrieved January 19, 2010, from A Dictionary of Biology website: http://www.encyclopedia.com
Drosophila melanogaster [ basic imformation about fruit flies]. (n.d.). Retrieved January 6, 2010, from http://en.wikipedia.org/wiki/Drosophila_melanogaster
Drosophila melanogaster. (n.d.). Retrieved January 6, 2010, from http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/Drosophila.html
Life Cycle of the Fruit Fly. (n.d.). Retrieved January 9, 2010, from Woodrow Wilson Biology Institute website: http://www.woodrow.org/teachers/bi/1994/life_cycle.html
Smoke. (n.d.). Retrieved January 8, 2010, from http://en.wikipedia.org/
Smoke. (n.d.). Retrieved January 17, 2010, from Merriam-Webster Online Dictionary. website: http://mw1.merriam-webster.com/dictionary/smoke
Toxic Smoke. (n.d.). Retrieved January 14, 2010, from PubMed U.S. National Library of Medicine website: http://www.ncbi.nlm.nih.gov/pubmed/3593498
This is my research paper:
Research Paper:
The topic that is being studied is the affect of air pollution on insect development. It is a very important issue that needs to be addressed. If air pollution has an affect on insects and their development, then it could affect humans, and also potentially the entire global ecosystem! This could happen easily if humans are not careful about air pollution. Though insects do not seem important compared to the rest of the world, they are the base of the food chain, and play a very important role in the ecosystem. This is because in an environment everything affects everything. If air pollution affects insect development, then animals that eat insects (such as frogs) could lose their food, and start to die out. Then animals that eat frogs (such snakes, lizards, birds, and small animals like hedge hogs) could lose food and start to die out, and so on. Everything affects everything in the food chain. A small problem could work its way up and wreck an entire ecosystem. If air pollution is causing a major problem with insects, then there could end up being a major problem with humans and the worldwide environment. That is why it is so important that the affect of air pollution on insects be studied.
The way to study this topic is by conducting an experiment that studies the affect of different levels of smoke on Drosophila melanogaster development. The independent variable will be different levels of smoke, which is a collection of airborne solid and liquid particles and gases that are emitted when combustion takes place. The make-up of smoke depends on what kind of a fuel is burning (“Smoke”, 2010). Smoke is a very good representation for air pollution, because it makes up many different kinds of air pollution, in particular black carbon air pollution, which is smoke from factories and other burning fuel (“Indoor and Outdoor Air Pollution”, 2010). The dependent variable will be the development of Drosophila melanogaster, otherwise known as fruit flies. Fruit flies are very common insects so they are a good representation of insects in this experiment. Fruit flies are small insects that have red eyes, a tan front portion of their body, and black rings across their back abdomen. They lay many eggs, breed quickly, are easy to take care of, and their life cycle is around 30 days when at 29 °C (84 °F) (“Drosophila melanogaster” 2010). Together these two variables would represent the affect of air pollution of insect development.
The two variables that are being studied are smoke, and fruit fly development. The dependent variable is fruit fly development. The scientific name for fruit fly is Drosophila melanogaster, and they are incredibly common insects. A fruit fly has four stages in life: egg, larva, pupa, and adult (“Life Cycle of a Fruit Fly”, 2010). Their nickname fruit fly originates from the fact they are often found around fruit that has been left alone, or is going bad. They do this because when a female fruit fly is ready to lay eggs, she lays them in moist fermenting food, or other organic decomposing materials. This is because once a larva (which is a baby insect) emerges from its egg; it will feed on the sugar from the material it was laid in, and also on the microorganisms that are decomposing that material (“Drosophila melanogaster” 2010). The development of a fruit fly is fairly simple. Once a fruit fly hatches, it goes through two molting periods, in which many parts are shed. Before and after these times of growth, a larva is called an instar, and there are three times when they are called that. After the third instar, the pauperism (which is the outer shell of the bug) becomes harder and darker than before. Within the pauperism is where the metamorphis takes place, which is when a larva turns into an adult. A person can tell when a fly is about to emerge from the puparium when it darkens. Once the metamorphis is completed, the new adult fly pushes it’s way out of the front end of the pauperism. Right after the adult fly emerges it is light with a long abdomen and folded wings, but a few hours later the fly turns darker, it spreads out its wings, and its abdomen becomes rounder. About 48 hours after a female fly emerges it can lay eggs, and the process begins again. A fly spends 8 days as an egg and in larval stages, then 6 days in the pupal stages. Its life span is several weeks and at normal room temperature (21 Co). A whole new population of fruit flies can be created in two weeks (“Life Cycle of a Fruit Fly”, 2010).
Varying levels of smoke is the independent variable. The definition of smoke is: “the gaseous products of burning materials especially of organic origin made visible by the presence of small particles of carbon” (“smoke”, 2010). To state that differently, it is the collection of airborne solid and liquid particles and gases. It occurs when a material undergoes combustion, and the nature of it depends on what is burning. Fires that have large amounts of oxygen around them burn at higher temperatures and they emit a smaller amount of smoke. Fires that burn with a lack of oxygen produce a much larger palette of compounds, and many of those compounds are toxic. Smoke can be visible or invisible to the naked eye, depending on the size of the particles. A good example of this is when you toast a piece of bread in a toaster. As the bread heats up, the products of combustion begin to get larger. The particles that are produced by the toast at first are invisible, but if the toast burns, the particles become visible. Smoke also can also be incredibly dangerous. In an average house fire for example, the smoke contains hundreds and hundreds of different chemicals and fumes. Usually the damage on the house is much greater from the smoke then from, the heat of the fire (“Smoke”, 2010).
The relationship between abiotic factors and biotic factors in the environment is very important. An abiotic factor is “Any of the nonliving factors that make up the abiotic environment in which living organisms occur.”(“abiotic factors”, 2004) To put it more simply, anything in an environment that is not living and didn’t come from something living is abiotic (for example, a nut is not abiotic because it comes from a tree). There are many examples of abiotic factors such as: temperature, weather, fire, gas, natural disasters (floods, earth quakes), elevation, UV rays, acid rain, humidity, air pollution, air pressure, the list goes on and on, and none of then come form something living. A biotic factor is “Any of the factors of an organism's environment that consist of other living organisms and together make up the biotic environment” (“biotic factors”, 2004) it is the opposite of an abiotic factor, because it is anything in an environment that is living and/or did come from something living (for example, a nut is biotic). Examples of biotic factors are: food (or lack of food), disease, predation, old age, injury, and so on. These two different kinds of factors are incredibly important in ecosystems.
In the experiment that is being conducted, smoke is the abiotic factor, and fruit fly development is the biotic factor. Just like in any environment, the abiotic factor affects the biotic factor. Some examples of this are when it is cold outside and a plant freezes and dies, or if there is not enough CO2 in the air and a plant dies because it cannot go through photosynthesis and produce glucose. Biotic factors are always affected by abiotic factors. In this experiment, the smoke affects the fruit fly development. This relationship is very important, because if smoke affects fruit flies it could end up affecting humans. That is because of the way the food chain works. First, stop being so specific and change the dependent variable of fruit flies to insects in general. Also, remember smoke is in many different kinds of air pollutions. Because of this, if smoke affects fruit flies and their development, then many different kinds of air pollution could affect insects and their development. If insects are affected and/or are developing differently due to air pollution, then their predators such as birds might lose a major source of food, because what they had been eating is no longer as common or has changed in a way that birds aren’t adapted to dealing with. Because of this lose of food birds and their survival rate could begin to diminish. Then animals that eat birds, like foxes, could lose their food source and their population could begin to diminish. Then the population of animals that eat foxes start to diminish, and its predator loses food, and so on and so on. It just works its way up the food chain, knocking everything out of balance. Eventually the entire global ecosystem could be trashed! So even though the change in insect development may seem like a minor problem, in the long run it can lead to a major issue.
This experiment is being studied for one reason. To figure out if air pollution has a major effect on ecosystems, and could eventually end up affecting the entire global eco-system. The independent variable is smoke, because smoke is a common component in air pollution, and it is a safe clean way to represent air pollution in an experiment. The dependent variable is fruit flies, because they are common insects that are easy to handle with large reproductive potential, and that is why they are good representatives for insects in general. If the smoke affects fruit fly development, then it can be assumed that air pollution can affect insect development. If insect development is compromised, then thousands of ecosystems could be destroyed! So, though it may not seem like it at first, the study of the affect of smoke on fruit fly development is incredibly important to our global ecosystem. That is why it is being studied.
References
“abiotic factor.” A Dictionary of Biology. 2004. Retrieved January 19, 2010 from E. (2004). abiotic factor. Retrieved January 19, 2010, from Encyclopedia.com database.
Air Pollution. (n.d.). Retrieved January 13, 2010, from Lawrence Berkeley National Laboratory website: http://www.lbl.gov/Education/ELSI/pollution-main.html
Atkinson, E. (2009, February 21). What is Smoke? Retrieved from http://chestofbooks.com/reference/The-New-Student-s-Reference-Work-Vol5/What-Is-Smoke.html
biotic factors. (2004). Retrieved January 19, 2010, from A Dictionary of Biology website: http://www.encyclopedia.com
Drosophila melanogaster [ basic imformation about fruit flies]. (n.d.). Retrieved January 6, 2010, from http://en.wikipedia.org/wiki/Drosophila_melanogaster
Drosophila melanogaster. (n.d.). Retrieved January 6, 2010, from http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/D/Drosophila.html
Life Cycle of the Fruit Fly. (n.d.). Retrieved January 9, 2010, from Woodrow Wilson Biology Institute website: http://www.woodrow.org/teachers/bi/1994/life_cycle.html
Smoke. (n.d.). Retrieved January 8, 2010, from http://en.wikipedia.org/
Smoke. (n.d.). Retrieved January 17, 2010, from Merriam-Webster Online Dictionary. website: http://mw1.merriam-webster.com/dictionary/smoke
Toxic Smoke. (n.d.). Retrieved January 14, 2010, from PubMed U.S. National Library of Medicine website: http://www.ncbi.nlm.nih.gov/pubmed/3593498
Wednesday, February 17, 2010
Today I worked on my experiment, and was able to get some photos of fruit fly wing structure. I also got some data. Here it is:
2/16/10 Control Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are hoping around a lot!!!
11 cc Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are not hoping around are as the control. there are less hatched flies
22 cc Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are not as active as the control. there are less hatched flies
33 cc Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are hoping not as much as control, and there is almost just as much hatched flies in the vial.
2/17/10 Control Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm There aren’t many puparium, and the oens that are there are darker so that means they’re about to emerge.
11 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm The flys are flying around, there are some puparium but they are lgiht
22 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm The flys are flying around, there are some puparium but they are lgiht
33 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm The flys are flying around, there are some puparium but they are lgiht
It doesn't allow e to post hte photos, so I can't.
2/16/10 Control Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are hoping around a lot!!!
11 cc Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are not hoping around are as the control. there are less hatched flies
22 cc Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are not as active as the control. there are less hatched flies
33 cc Puparium: 3 mm
Adult: 3 mm
Larva: 5 mm The flies are hoping not as much as control, and there is almost just as much hatched flies in the vial.
2/17/10 Control Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm There aren’t many puparium, and the oens that are there are darker so that means they’re about to emerge.
11 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm The flys are flying around, there are some puparium but they are lgiht
22 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm The flys are flying around, there are some puparium but they are lgiht
33 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm The flys are flying around, there are some puparium but they are lgiht
It doesn't allow e to post hte photos, so I can't.
Thursday, February 11, 2010
Thursday Febuary 11th
It was another snow day today. We're driving up to Vermont today. If i have time, i might work on my research paper on the drive up.
Wednesday, February 10, 2010
Wensday, febuary 10th 2010
Today was a snowday, so i could not work on my project.
I did not work on my research paper today. I will work on it over the long weekend.
I did not work on my research paper today. I will work on it over the long weekend.
Tuesday, Febuary 9th 2010
Today I did my experiment, and happily learned that my bugs have hatched! surprisingly, the experiment is going like i predicted, and the bugs in the vials with the most smoke have not hatched! I'm so happy that my experiment is ACTUALLY working!
this is the data i collected today:
2/9/10 control Adult flies: around 3 or 2.5 mm
Flies in puparium:
3 mm
larva: 5 mm Some larva have hatched, and some have gone into the puparium and are undergoing metemorphis. From what I can see 7 new adlut flies have hatched.
Tgere are many in the puparium, but they are to hard ot count.
There are still some larva left.
11 cc Adult: 3 mm
Puparium: 3 mm
Larva 5 mm 6 flies have under gone matamorphis and have emerged as an adult fly. There are still some in the puparium though.
I saw 1 larva moving around, so I assume there is more.
22 cc Adult: 3 mm
Puparium: 2-3 mm
Larva: 5 mm So far 1 fly has emerged, the rest are in the puparium. A few of the pupariums have darkens, so I know the dult fly will emerge soon.
33 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm 1 fruit fly has emerged, and the rest are in the puparium. Once again, a few of the pupariums have darkened so I know flys will emerge soon.
this is the data i collected today:
2/9/10 control Adult flies: around 3 or 2.5 mm
Flies in puparium:
3 mm
larva: 5 mm Some larva have hatched, and some have gone into the puparium and are undergoing metemorphis. From what I can see 7 new adlut flies have hatched.
Tgere are many in the puparium, but they are to hard ot count.
There are still some larva left.
11 cc Adult: 3 mm
Puparium: 3 mm
Larva 5 mm 6 flies have under gone matamorphis and have emerged as an adult fly. There are still some in the puparium though.
I saw 1 larva moving around, so I assume there is more.
22 cc Adult: 3 mm
Puparium: 2-3 mm
Larva: 5 mm So far 1 fly has emerged, the rest are in the puparium. A few of the pupariums have darkens, so I know the dult fly will emerge soon.
33 cc Adult: 3 mm
Puparium: 3 mm
Larva: 5 mm 1 fruit fly has emerged, and the rest are in the puparium. Once again, a few of the pupariums have darkened so I know flys will emerge soon.
Monday, February 8, 2010
Monday, Fevuary 8th 2010
Today I stayed home form school, so i was not able to subject my fruit flies to smoke. This really worries me, because i wasn't able to do it during weekends either. I'm afraid my data will not come out the way i expected it to because of all these skipped days of doing data.
I worked on my research paper today, but because I was sick (i spent most of the day sleeping) all i could d was edit what i had done the day before.
I would like to go see ms. Weissman tomorrow because I have a lot of questions about my research paper.
I worked on my research paper today, but because I was sick (i spent most of the day sleeping) all i could d was edit what i had done the day before.
I would like to go see ms. Weissman tomorrow because I have a lot of questions about my research paper.
Friday, February 5, 2010
Sunday, febuary 7th 2010
Today I worked on my research paper. I completely rewrote my introduction, and made it two separate paragraphs. The first is about what topic i am studying and it's importance (like ms. Weissman said to do). The next one goes into specifics, and a lot of it is my old introduction. This is my new first paragraph:
The topic that is wished to be studied is the affect of air pollution on insects. It is a very important issue that needs to be addressed. If air pollution haves an affect on insects and their development, then it could affect humans, and also the entire global ecosystem! This could happen very simply in one way. Though insects do not seem important right now, they are the base of the food chain, and play a very important role in the ecosystem. This is because in an environment everything affects everything. If air pollution affects insect development, then animals that eat insects (such as frogs) could lose their food, and start to die out. Then animals that eat frogs (such snakes, lizards, birds, and small animals like hedge hogs) could lose food and start to die out, and so on. Everything affects everything in the food chain. A small problem could work it’s way up and wreck an entire ecosystem. If air pollution is causing a major problem with insects, then there could end up being a major problem with humans and the worldwide environment. That is why it is so important that the affect of air pollution on insects be studied.
The topic that is wished to be studied is the affect of air pollution on insects. It is a very important issue that needs to be addressed. If air pollution haves an affect on insects and their development, then it could affect humans, and also the entire global ecosystem! This could happen very simply in one way. Though insects do not seem important right now, they are the base of the food chain, and play a very important role in the ecosystem. This is because in an environment everything affects everything. If air pollution affects insect development, then animals that eat insects (such as frogs) could lose their food, and start to die out. Then animals that eat frogs (such snakes, lizards, birds, and small animals like hedge hogs) could lose food and start to die out, and so on. Everything affects everything in the food chain. A small problem could work it’s way up and wreck an entire ecosystem. If air pollution is causing a major problem with insects, then there could end up being a major problem with humans and the worldwide environment. That is why it is so important that the affect of air pollution on insects be studied.
Thursday, February 4, 2010
Thursday Febuary 4th
today I did my experiment i walked into the class room. I walked in, got goggles and a box of matches, and light hte candles. Then I waited for 10 minutes, and then added the smoke to the vials. I really hope my experiment will work. I'm also not sure about my experimental design.
I am very very confused about ms. Weissman's comments on my Reeach paper. I am going to see her after school. First she helped me with my data table, and it ended up looking like this:
Date Amount of Smoke Average Length of Larva/Adult Observations
2/3/10 control 5 mm
11 cc 5 mm
22 cc 5 mm
33 cc 5 mm
2/4/10 control 5 mm They seems to be not a lot of larva in the control
11 cc 5 mm They are moving around and eating
22 cc 5 mm Many are moving around and eating
33 cc 5 mm Many are moving around and eating
Then we went over my research paper and i have all my questions answered. Ithink i have a basic idea of what i want to do.
I am very very confused about ms. Weissman's comments on my Reeach paper. I am going to see her after school. First she helped me with my data table, and it ended up looking like this:
Date Amount of Smoke Average Length of Larva/Adult Observations
2/3/10 control 5 mm
11 cc 5 mm
22 cc 5 mm
33 cc 5 mm
2/4/10 control 5 mm They seems to be not a lot of larva in the control
11 cc 5 mm They are moving around and eating
22 cc 5 mm Many are moving around and eating
33 cc 5 mm Many are moving around and eating
Then we went over my research paper and i have all my questions answered. Ithink i have a basic idea of what i want to do.
Wednesday, February 3, 2010
Wensday febuary 3rd
Today I started my experiment. I learned many things that I need to change about my experiment set up. First off, I need a lighter instead of matches, because it takes up to many matches to light the candles, plus to light the candles i have to point the match down, and then the fire touches my fingers and burns me. I also learned I'm going to have to bring in more candles, because the small tea ones easily break. I ALSO learned that I need to let the smoke mingle for 10 minutes, because after 3 minutes the smoke does not mingle enough.
I measured my fruit fly larva, they are 5 mm long.
I have some doubts about weather my experiment is going t work, but I feel like i should stick with it, so that is what i will do. I will see if they are affected.
I measured my fruit fly larva, they are 5 mm long.
I have some doubts about weather my experiment is going t work, but I feel like i should stick with it, so that is what i will do. I will see if they are affected.
Wednesday, January 27, 2010
Wensday january 27th
Today I typed up all of my experimental design that i have so far. That only problem i'm having is my data table, and what data i will observe.
Tuesday january 26th!
Today i set u my fruit fly set up. this is what i did:
Procedure:
1. take the ice pick, and gab a hole big enough for a syringe tip in the top and the side of the metal box.
2. put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. repeat 3 times
6. take the same graduated cylinder and fill it with 14 mL of water.
7. take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the mixture a very dark blue.
9. pour the blue mixture into the same vial with the fruit fly media.
10. repeat 3 times, pouring the mixture over the vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
Procedure:
1. take the ice pick, and gab a hole big enough for a syringe tip in the top and the side of the metal box.
2. put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. repeat 3 times
6. take the same graduated cylinder and fill it with 14 mL of water.
7. take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the mixture a very dark blue.
9. pour the blue mixture into the same vial with the fruit fly media.
10. repeat 3 times, pouring the mixture over the vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
Monday, January 25, 2010
Monday! January 25 2010!!!!!!
I learned form seeing Ms. Weissman that i need a pragraph that restated my paper. I wrote it, and it's 156 words long:
This experiment is being studied for one reason. To figure out if air pollution has a major affect on ecosystems, and could eventually end up affecting the entire global eco-system. The independent variable is smoke, because smoke is a common component in air pollution, and it is a safe clean way to represent air pollution in an experiment. The dependent variable is fruit flies, because they are common insects that are easy to handle with large reproductive potential, and that is why they are good representatives for insects in general. If the smoke affects fruit fly development, then it can be assumed that air pollution can affect insect development. If insect development is compromised, then thousands of ecosystems could be destroyed! So, though it may not seems like it at first, the study of the affect of smoke on fruit fly development is incredibly important to our global ecosystem. That is why it is being studied.
I also have decided that i would like 4 vials of fies. one that is a control (no smoke added) one that i add a small amount of smoke to, one i add a medium amount of smoke to, and one i add a large amount of smoke to.
This experiment is being studied for one reason. To figure out if air pollution has a major affect on ecosystems, and could eventually end up affecting the entire global eco-system. The independent variable is smoke, because smoke is a common component in air pollution, and it is a safe clean way to represent air pollution in an experiment. The dependent variable is fruit flies, because they are common insects that are easy to handle with large reproductive potential, and that is why they are good representatives for insects in general. If the smoke affects fruit fly development, then it can be assumed that air pollution can affect insect development. If insect development is compromised, then thousands of ecosystems could be destroyed! So, though it may not seems like it at first, the study of the affect of smoke on fruit fly development is incredibly important to our global ecosystem. That is why it is being studied.
I also have decided that i would like 4 vials of fies. one that is a control (no smoke added) one that i add a small amount of smoke to, one i add a medium amount of smoke to, and one i add a large amount of smoke to.
Sunday, January 24, 2010
Sunday
I am proof reading my research paper but i think i'm done. i'm working ideas for experimental set up, i think i've answered all the questions.
my big picture procedure:
I am first going to look at normal fruit flies not subjected to smoke. then put them in the container, subject them to smoke, and let them breed
i will look at the next generation of furit flies, see of they have deformities, if they take a longer period of time to develop.
them i will breed that generation of fruit flies, and see if the trait is passed on.
In order to know the volume of the smoke, i will need to learn the volume o the vile, and then i will see how much i add on.
then i can calculate the volume
I am looking at the basic structure of a fruit fly (color, if the wings look normal, stuff like that) then once i have a new generation of larva subjected to smoke, i will study how long it takes them to develop.
to make media: 15 mL of drasophila media, and 15 mL of water
I think i want winged
my big picture procedure:
I am first going to look at normal fruit flies not subjected to smoke. then put them in the container, subject them to smoke, and let them breed
i will look at the next generation of furit flies, see of they have deformities, if they take a longer period of time to develop.
them i will breed that generation of fruit flies, and see if the trait is passed on.
In order to know the volume of the smoke, i will need to learn the volume o the vile, and then i will see how much i add on.
then i can calculate the volume
I am looking at the basic structure of a fruit fly (color, if the wings look normal, stuff like that) then once i have a new generation of larva subjected to smoke, i will study how long it takes them to develop.
to make media: 15 mL of drasophila media, and 15 mL of water
I think i want winged
Wednesday, January 20, 2010
Wensday january 20th
I saw ms. weissmen and learned that i don't need to have a seprate parapgraph for why my subject is important, because i had already done it.
Tuesday, January 19, 2010
Tuesday
Today i saw ms. Weissmen. I learned i need to learn about abiotic factor and include it in my paper.
I'm starting work on my 4th paragraph.
I'm starting work on my 4th paragraph.
Monday, January 18, 2010
Monday!
Even though it is a no homework weekend i worked a little bit on my paper today. I'm going to work on some more later. I have been having trouble find sites ot research smoke, but i havel ots of good research on furit flies.
Friday, January 15, 2010
Friday, january 15th
I went to go see ms. weissman, and relized that for my secound paragraph i need to write about the relationship of biotic factors and a biotic factors.
Thursday, January 14, 2010
Thursday, January 13th
Ms. Weissmen wasn't here today I think I have done a lot of research, so I am going start writing my Research paper, so i will know what i need to research.
I found this site that has good information on smoke: http://chestofbooks.com/reference/The-New-Student-s-Reference-Work-Vol5/What-Is-Smoke.html
I'm not sure how to do intext citations with web sites so i'm going ot wait to put them in.
I found this site that has good information on smoke: http://chestofbooks.com/reference/The-New-Student-s-Reference-Work-Vol5/What-Is-Smoke.html
I'm not sure how to do intext citations with web sites so i'm going ot wait to put them in.
Wednesday, January 13, 2010
Wensday.
Today I start working on my research paper. I already had a lot of info on fruit flies, so what I am focusing on is Air polution.
the notes i have so far on fruit flies are:
Fruit flies:
• Adults are about 1/8 inch long and usually have red eyes.
• The front portion of the body is tan and the rear portion is black.
• Fruit flies lay their eggs near the surface of fermenting foods or other moist, organic materials. Once they emerge the larvae continue to feed on the moist organic food
• They have a very large reproductive potential, they can lay 500 eggs if they are given the chance
• The life cycle from egg to adult is in around a week.
• A new generation of adult flies can be created in 2 weeks.
Wiki:
The species is commonly known as the common fruit fly or vinegar fly. Starting from Charles W. Woodworth, this species is one of the most commonly used model organisms in biology, including studies in genetics, physiology, microbial pathogenesis and life history evolution because they are easy to take care of, breed quickly, and lay many eggs.[3]
Appearance
• Fruit flies have red eyes
• And have and have horizontal black rings across their abdomen
• Their appearance changes die to their sex, which is called sexual dimorphism.
o The females are around 2.5 millimeters long, and males are a tiny bit smaller, and the back of their bodies is darker
o Males can be easily identified out of a crowd of females, because if their color difference, which is a specific black areas at their abdomen
Development:
• The period of time in which a fruit fly develops changes due to the temperature
• The temperature of the area that the fruit fly is incredibly important because as it determines how fast the fruit fly will develop. If it is:
• 28 °C (82 °F) then the egg can develop in 7 days
• 30 °C (86 °F), then the egg can take 11 days because of heat stress
• 18 °C (64 °) then the egg could take 19 days
• 12 °C (54 °F) then the egg could take over 50 days
• The best possible temperature is 25 °C (77 °F) and at that time the fruit flies take 8.5 days to develop
• Females lay around 400 eggs (which are called embryo) about five at a time.
• They lay these eggs into soft decaying materials, like rotting mushrooms and rotting fruit
• The eggs are around .5 millimeters long
• They hatch after12-15 hours (this is measured at the ideal temperature of 25°C
• The larvae that are hatched grow for around 4 days (at that same 25 °C)
• They molt (or shed of their outer layer of their body) two times, turning them into 2nd- and then 3rd- instar larvae, after about 24 and 48 hours after they are hatched
• During this time period they eat microorganisms that decompose the rotting fruit, and also the sugar form the fruit it’s self.
• The larvae then go through a 4 day long metamorphosis (still at that same 25°C), and after that the adults emerge
• Females are ready to court males 8-12 hours after they emerge as adults.
Flight:
• The wings of a fly can beat up to 220 times per second.
•
From users.rcn.com:
Body:
• And insect has a
o Head,
o Thorax,
• The thorax has 3 different segments (T1, T2, T2) and they each have 2 legs, resulting in a six legged insect
o And abdomen.
• Most insects have wings on T2 and T3 but on a fruit fly there is only one pair of wings, on T2
From 1994 Woodrow Wilson Biology Institute
• A fruit fly has 4 stages in their life cycle
o Egg
o Larva
o Pupa
o And adult
• At typical class room temperature (which is 21 C) a new fruit fly population of adults can be produced in two weeks
o 8 days being an egg and in larval stages and 6 days in the pupal stage.
o The life span of a fruit fly could be several weeks
Life cycle:
• Twenty for hours after an egg is laid, the larva hatches (larva is a baby bug)
• The larva has 2 molting periods, in which many parts are shed, including the cuticle, mouth, hooks, and spiracles are shed.
o Larva is called an instar during the periods of time before and after growth. Fruit flies have three
• The puparium (the hard outer shell of the bug) from the third instar develops and becomes hard and dark.
The puparium is where metamorphosis takes place. Right before the adult fly comes out of the puparium, it darkens.
• About 24 hours before the adult fly emerges, you can see folded wings and eyes if you look closely
o Once metamorphosis is complete, the adult fly pushes its way out our the front end of the piparium.
o First the fruit fly is light with a long abdomen with folded wings, but after a few hours the fly turns darker, the abdomen gets rounder, and the fly speads out it’s wings
• After about 48 hours after the fly comes out of the puparium, females can begin to lay eggs.
o
the notes i have so far on fruit flies are:
Fruit flies:
• Adults are about 1/8 inch long and usually have red eyes.
• The front portion of the body is tan and the rear portion is black.
• Fruit flies lay their eggs near the surface of fermenting foods or other moist, organic materials. Once they emerge the larvae continue to feed on the moist organic food
• They have a very large reproductive potential, they can lay 500 eggs if they are given the chance
• The life cycle from egg to adult is in around a week.
• A new generation of adult flies can be created in 2 weeks.
Wiki:
The species is commonly known as the common fruit fly or vinegar fly. Starting from Charles W. Woodworth, this species is one of the most commonly used model organisms in biology, including studies in genetics, physiology, microbial pathogenesis and life history evolution because they are easy to take care of, breed quickly, and lay many eggs.[3]
Appearance
• Fruit flies have red eyes
• And have and have horizontal black rings across their abdomen
• Their appearance changes die to their sex, which is called sexual dimorphism.
o The females are around 2.5 millimeters long, and males are a tiny bit smaller, and the back of their bodies is darker
o Males can be easily identified out of a crowd of females, because if their color difference, which is a specific black areas at their abdomen
Development:
• The period of time in which a fruit fly develops changes due to the temperature
• The temperature of the area that the fruit fly is incredibly important because as it determines how fast the fruit fly will develop. If it is:
• 28 °C (82 °F) then the egg can develop in 7 days
• 30 °C (86 °F), then the egg can take 11 days because of heat stress
• 18 °C (64 °) then the egg could take 19 days
• 12 °C (54 °F) then the egg could take over 50 days
• The best possible temperature is 25 °C (77 °F) and at that time the fruit flies take 8.5 days to develop
• Females lay around 400 eggs (which are called embryo) about five at a time.
• They lay these eggs into soft decaying materials, like rotting mushrooms and rotting fruit
• The eggs are around .5 millimeters long
• They hatch after12-15 hours (this is measured at the ideal temperature of 25°C
• The larvae that are hatched grow for around 4 days (at that same 25 °C)
• They molt (or shed of their outer layer of their body) two times, turning them into 2nd- and then 3rd- instar larvae, after about 24 and 48 hours after they are hatched
• During this time period they eat microorganisms that decompose the rotting fruit, and also the sugar form the fruit it’s self.
• The larvae then go through a 4 day long metamorphosis (still at that same 25°C), and after that the adults emerge
• Females are ready to court males 8-12 hours after they emerge as adults.
Flight:
• The wings of a fly can beat up to 220 times per second.
•
From users.rcn.com:
Body:
• And insect has a
o Head,
o Thorax,
• The thorax has 3 different segments (T1, T2, T2) and they each have 2 legs, resulting in a six legged insect
o And abdomen.
• Most insects have wings on T2 and T3 but on a fruit fly there is only one pair of wings, on T2
From 1994 Woodrow Wilson Biology Institute
• A fruit fly has 4 stages in their life cycle
o Egg
o Larva
o Pupa
o And adult
• At typical class room temperature (which is 21 C) a new fruit fly population of adults can be produced in two weeks
o 8 days being an egg and in larval stages and 6 days in the pupal stage.
o The life span of a fruit fly could be several weeks
Life cycle:
• Twenty for hours after an egg is laid, the larva hatches (larva is a baby bug)
• The larva has 2 molting periods, in which many parts are shed, including the cuticle, mouth, hooks, and spiracles are shed.
o Larva is called an instar during the periods of time before and after growth. Fruit flies have three
• The puparium (the hard outer shell of the bug) from the third instar develops and becomes hard and dark.
The puparium is where metamorphosis takes place. Right before the adult fly comes out of the puparium, it darkens.
• About 24 hours before the adult fly emerges, you can see folded wings and eyes if you look closely
o Once metamorphosis is complete, the adult fly pushes its way out our the front end of the piparium.
o First the fruit fly is light with a long abdomen with folded wings, but after a few hours the fly turns darker, the abdomen gets rounder, and the fly speads out it’s wings
• After about 48 hours after the fly comes out of the puparium, females can begin to lay eggs.
o
Tuesday
I posted my application, and I feel good about it. Now I need to start my research paper, but I'm worried about how hard it will be.
Tuesday, January 12, 2010
Monday
I am almost completely done with my application form, all I need to do is change my procedure to make sure that I don't burn all my fruit flies.
Sunday, January 10, 2010
Sunday
I still can't figure out how i am supposed to write about how my two variables relate, but other then that I am done with my application. I emailed ms. weissmen a couple times.
Saturday, January 9, 2010
Saturday
I began to work on my application form, but I am very confused on what to write, I've been asking around, but no else seems to know either. I posted to Form on moodle, and Finally know what my experimental question is, what my hypothesis is, and what my variables are.
I still wish i knew what to write for my application though.
Friday, January 8, 2010
I KNOW MY TOPIC!
Today I went to go see Mrs. Weissmen to make sure I knew what I was doing, and what my topic was. I do now!!! It is the the effect f smoke on the development of fruit flies
Thursday, January 7, 2010
Wednesday, January 6, 2010
Question Template
how does X affect Y?
at what point does X affect Y?
What are the implications of X to Y?
At what point does X not affect Y?
Why does X affect Y?
X= independent
y= dependent
I saw Mrs. Weissman today, and I learned that i just need to research more, especially car exhaust and and cigarette smoke. I need to learn about the DEVELOPMENT OF FRUIT FLIES!!!!!!
at what point does X affect Y?
What are the implications of X to Y?
At what point does X not affect Y?
Why does X affect Y?
X= independent
y= dependent
I saw Mrs. Weissman today, and I learned that i just need to research more, especially car exhaust and and cigarette smoke. I need to learn about the DEVELOPMENT OF FRUIT FLIES!!!!!!
Tuesday, January 5, 2010
list of factors
Abiotic factors:
water, or drought and lack of wawter
temperture extremes
weather
light
fire
gases
rock
snow
land slide
natural disasters: flood, earth quakes
elevation
climate
lack of space
lack of shelter
salinity
UV rays
Sun light
acid rain
humidity
airpressure
air pollution
soil
water polution
radiation
electricity magnetism
electromagnetism
Biotic factor:
food, or lack of food
disease
predation
old age
ingury
water, or drought and lack of wawter
temperture extremes
weather
light
fire
gases
rock
snow
land slide
natural disasters: flood, earth quakes
elevation
climate
lack of space
lack of shelter
salinity
UV rays
Sun light
acid rain
humidity
airpressure
air pollution
soil
water polution
radiation
electricity magnetism
electromagnetism
Biotic factor:
food, or lack of food
disease
predation
old age
ingury
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