Wednesday, January 27, 2010
Wensday january 27th
Today I typed up all of my experimental design that i have so far. That only problem i'm having is my data table, and what data i will observe.
Tuesday january 26th!
Today i set u my fruit fly set up. this is what i did:
Procedure:
1. take the ice pick, and gab a hole big enough for a syringe tip in the top and the side of the metal box.
2. put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. repeat 3 times
6. take the same graduated cylinder and fill it with 14 mL of water.
7. take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the mixture a very dark blue.
9. pour the blue mixture into the same vial with the fruit fly media.
10. repeat 3 times, pouring the mixture over the vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
Procedure:
1. take the ice pick, and gab a hole big enough for a syringe tip in the top and the side of the metal box.
2. put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. repeat 3 times
6. take the same graduated cylinder and fill it with 14 mL of water.
7. take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the mixture a very dark blue.
9. pour the blue mixture into the same vial with the fruit fly media.
10. repeat 3 times, pouring the mixture over the vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
Monday, January 25, 2010
Monday! January 25 2010!!!!!!
I learned form seeing Ms. Weissman that i need a pragraph that restated my paper. I wrote it, and it's 156 words long:
This experiment is being studied for one reason. To figure out if air pollution has a major affect on ecosystems, and could eventually end up affecting the entire global eco-system. The independent variable is smoke, because smoke is a common component in air pollution, and it is a safe clean way to represent air pollution in an experiment. The dependent variable is fruit flies, because they are common insects that are easy to handle with large reproductive potential, and that is why they are good representatives for insects in general. If the smoke affects fruit fly development, then it can be assumed that air pollution can affect insect development. If insect development is compromised, then thousands of ecosystems could be destroyed! So, though it may not seems like it at first, the study of the affect of smoke on fruit fly development is incredibly important to our global ecosystem. That is why it is being studied.
I also have decided that i would like 4 vials of fies. one that is a control (no smoke added) one that i add a small amount of smoke to, one i add a medium amount of smoke to, and one i add a large amount of smoke to.
This experiment is being studied for one reason. To figure out if air pollution has a major affect on ecosystems, and could eventually end up affecting the entire global eco-system. The independent variable is smoke, because smoke is a common component in air pollution, and it is a safe clean way to represent air pollution in an experiment. The dependent variable is fruit flies, because they are common insects that are easy to handle with large reproductive potential, and that is why they are good representatives for insects in general. If the smoke affects fruit fly development, then it can be assumed that air pollution can affect insect development. If insect development is compromised, then thousands of ecosystems could be destroyed! So, though it may not seems like it at first, the study of the affect of smoke on fruit fly development is incredibly important to our global ecosystem. That is why it is being studied.
I also have decided that i would like 4 vials of fies. one that is a control (no smoke added) one that i add a small amount of smoke to, one i add a medium amount of smoke to, and one i add a large amount of smoke to.
Sunday, January 24, 2010
Sunday
I am proof reading my research paper but i think i'm done. i'm working ideas for experimental set up, i think i've answered all the questions.
my big picture procedure:
I am first going to look at normal fruit flies not subjected to smoke. then put them in the container, subject them to smoke, and let them breed
i will look at the next generation of furit flies, see of they have deformities, if they take a longer period of time to develop.
them i will breed that generation of fruit flies, and see if the trait is passed on.
In order to know the volume of the smoke, i will need to learn the volume o the vile, and then i will see how much i add on.
then i can calculate the volume
I am looking at the basic structure of a fruit fly (color, if the wings look normal, stuff like that) then once i have a new generation of larva subjected to smoke, i will study how long it takes them to develop.
to make media: 15 mL of drasophila media, and 15 mL of water
I think i want winged
my big picture procedure:
I am first going to look at normal fruit flies not subjected to smoke. then put them in the container, subject them to smoke, and let them breed
i will look at the next generation of furit flies, see of they have deformities, if they take a longer period of time to develop.
them i will breed that generation of fruit flies, and see if the trait is passed on.
In order to know the volume of the smoke, i will need to learn the volume o the vile, and then i will see how much i add on.
then i can calculate the volume
I am looking at the basic structure of a fruit fly (color, if the wings look normal, stuff like that) then once i have a new generation of larva subjected to smoke, i will study how long it takes them to develop.
to make media: 15 mL of drasophila media, and 15 mL of water
I think i want winged
Wednesday, January 20, 2010
Wensday january 20th
I saw ms. weissmen and learned that i don't need to have a seprate parapgraph for why my subject is important, because i had already done it.
Tuesday, January 19, 2010
Tuesday
Today i saw ms. Weissmen. I learned i need to learn about abiotic factor and include it in my paper.
I'm starting work on my 4th paragraph.
I'm starting work on my 4th paragraph.
Monday, January 18, 2010
Monday!
Even though it is a no homework weekend i worked a little bit on my paper today. I'm going to work on some more later. I have been having trouble find sites ot research smoke, but i havel ots of good research on furit flies.
Friday, January 15, 2010
Friday, january 15th
I went to go see ms. weissman, and relized that for my secound paragraph i need to write about the relationship of biotic factors and a biotic factors.
Thursday, January 14, 2010
Thursday, January 13th
Ms. Weissmen wasn't here today I think I have done a lot of research, so I am going start writing my Research paper, so i will know what i need to research.
I found this site that has good information on smoke: http://chestofbooks.com/reference/The-New-Student-s-Reference-Work-Vol5/What-Is-Smoke.html
I'm not sure how to do intext citations with web sites so i'm going ot wait to put them in.
I found this site that has good information on smoke: http://chestofbooks.com/reference/The-New-Student-s-Reference-Work-Vol5/What-Is-Smoke.html
I'm not sure how to do intext citations with web sites so i'm going ot wait to put them in.
Wednesday, January 13, 2010
Wensday.
Today I start working on my research paper. I already had a lot of info on fruit flies, so what I am focusing on is Air polution.
the notes i have so far on fruit flies are:
Fruit flies:
• Adults are about 1/8 inch long and usually have red eyes.
• The front portion of the body is tan and the rear portion is black.
• Fruit flies lay their eggs near the surface of fermenting foods or other moist, organic materials. Once they emerge the larvae continue to feed on the moist organic food
• They have a very large reproductive potential, they can lay 500 eggs if they are given the chance
• The life cycle from egg to adult is in around a week.
• A new generation of adult flies can be created in 2 weeks.
Wiki:
The species is commonly known as the common fruit fly or vinegar fly. Starting from Charles W. Woodworth, this species is one of the most commonly used model organisms in biology, including studies in genetics, physiology, microbial pathogenesis and life history evolution because they are easy to take care of, breed quickly, and lay many eggs.[3]
Appearance
• Fruit flies have red eyes
• And have and have horizontal black rings across their abdomen
• Their appearance changes die to their sex, which is called sexual dimorphism.
o The females are around 2.5 millimeters long, and males are a tiny bit smaller, and the back of their bodies is darker
o Males can be easily identified out of a crowd of females, because if their color difference, which is a specific black areas at their abdomen
Development:
• The period of time in which a fruit fly develops changes due to the temperature
• The temperature of the area that the fruit fly is incredibly important because as it determines how fast the fruit fly will develop. If it is:
• 28 °C (82 °F) then the egg can develop in 7 days
• 30 °C (86 °F), then the egg can take 11 days because of heat stress
• 18 °C (64 °) then the egg could take 19 days
• 12 °C (54 °F) then the egg could take over 50 days
• The best possible temperature is 25 °C (77 °F) and at that time the fruit flies take 8.5 days to develop
• Females lay around 400 eggs (which are called embryo) about five at a time.
• They lay these eggs into soft decaying materials, like rotting mushrooms and rotting fruit
• The eggs are around .5 millimeters long
• They hatch after12-15 hours (this is measured at the ideal temperature of 25°C
• The larvae that are hatched grow for around 4 days (at that same 25 °C)
• They molt (or shed of their outer layer of their body) two times, turning them into 2nd- and then 3rd- instar larvae, after about 24 and 48 hours after they are hatched
• During this time period they eat microorganisms that decompose the rotting fruit, and also the sugar form the fruit it’s self.
• The larvae then go through a 4 day long metamorphosis (still at that same 25°C), and after that the adults emerge
• Females are ready to court males 8-12 hours after they emerge as adults.
Flight:
• The wings of a fly can beat up to 220 times per second.
•
From users.rcn.com:
Body:
• And insect has a
o Head,
o Thorax,
• The thorax has 3 different segments (T1, T2, T2) and they each have 2 legs, resulting in a six legged insect
o And abdomen.
• Most insects have wings on T2 and T3 but on a fruit fly there is only one pair of wings, on T2
From 1994 Woodrow Wilson Biology Institute
• A fruit fly has 4 stages in their life cycle
o Egg
o Larva
o Pupa
o And adult
• At typical class room temperature (which is 21 C) a new fruit fly population of adults can be produced in two weeks
o 8 days being an egg and in larval stages and 6 days in the pupal stage.
o The life span of a fruit fly could be several weeks
Life cycle:
• Twenty for hours after an egg is laid, the larva hatches (larva is a baby bug)
• The larva has 2 molting periods, in which many parts are shed, including the cuticle, mouth, hooks, and spiracles are shed.
o Larva is called an instar during the periods of time before and after growth. Fruit flies have three
• The puparium (the hard outer shell of the bug) from the third instar develops and becomes hard and dark.
The puparium is where metamorphosis takes place. Right before the adult fly comes out of the puparium, it darkens.
• About 24 hours before the adult fly emerges, you can see folded wings and eyes if you look closely
o Once metamorphosis is complete, the adult fly pushes its way out our the front end of the piparium.
o First the fruit fly is light with a long abdomen with folded wings, but after a few hours the fly turns darker, the abdomen gets rounder, and the fly speads out it’s wings
• After about 48 hours after the fly comes out of the puparium, females can begin to lay eggs.
o
the notes i have so far on fruit flies are:
Fruit flies:
• Adults are about 1/8 inch long and usually have red eyes.
• The front portion of the body is tan and the rear portion is black.
• Fruit flies lay their eggs near the surface of fermenting foods or other moist, organic materials. Once they emerge the larvae continue to feed on the moist organic food
• They have a very large reproductive potential, they can lay 500 eggs if they are given the chance
• The life cycle from egg to adult is in around a week.
• A new generation of adult flies can be created in 2 weeks.
Wiki:
The species is commonly known as the common fruit fly or vinegar fly. Starting from Charles W. Woodworth, this species is one of the most commonly used model organisms in biology, including studies in genetics, physiology, microbial pathogenesis and life history evolution because they are easy to take care of, breed quickly, and lay many eggs.[3]
Appearance
• Fruit flies have red eyes
• And have and have horizontal black rings across their abdomen
• Their appearance changes die to their sex, which is called sexual dimorphism.
o The females are around 2.5 millimeters long, and males are a tiny bit smaller, and the back of their bodies is darker
o Males can be easily identified out of a crowd of females, because if their color difference, which is a specific black areas at their abdomen
Development:
• The period of time in which a fruit fly develops changes due to the temperature
• The temperature of the area that the fruit fly is incredibly important because as it determines how fast the fruit fly will develop. If it is:
• 28 °C (82 °F) then the egg can develop in 7 days
• 30 °C (86 °F), then the egg can take 11 days because of heat stress
• 18 °C (64 °) then the egg could take 19 days
• 12 °C (54 °F) then the egg could take over 50 days
• The best possible temperature is 25 °C (77 °F) and at that time the fruit flies take 8.5 days to develop
• Females lay around 400 eggs (which are called embryo) about five at a time.
• They lay these eggs into soft decaying materials, like rotting mushrooms and rotting fruit
• The eggs are around .5 millimeters long
• They hatch after12-15 hours (this is measured at the ideal temperature of 25°C
• The larvae that are hatched grow for around 4 days (at that same 25 °C)
• They molt (or shed of their outer layer of their body) two times, turning them into 2nd- and then 3rd- instar larvae, after about 24 and 48 hours after they are hatched
• During this time period they eat microorganisms that decompose the rotting fruit, and also the sugar form the fruit it’s self.
• The larvae then go through a 4 day long metamorphosis (still at that same 25°C), and after that the adults emerge
• Females are ready to court males 8-12 hours after they emerge as adults.
Flight:
• The wings of a fly can beat up to 220 times per second.
•
From users.rcn.com:
Body:
• And insect has a
o Head,
o Thorax,
• The thorax has 3 different segments (T1, T2, T2) and they each have 2 legs, resulting in a six legged insect
o And abdomen.
• Most insects have wings on T2 and T3 but on a fruit fly there is only one pair of wings, on T2
From 1994 Woodrow Wilson Biology Institute
• A fruit fly has 4 stages in their life cycle
o Egg
o Larva
o Pupa
o And adult
• At typical class room temperature (which is 21 C) a new fruit fly population of adults can be produced in two weeks
o 8 days being an egg and in larval stages and 6 days in the pupal stage.
o The life span of a fruit fly could be several weeks
Life cycle:
• Twenty for hours after an egg is laid, the larva hatches (larva is a baby bug)
• The larva has 2 molting periods, in which many parts are shed, including the cuticle, mouth, hooks, and spiracles are shed.
o Larva is called an instar during the periods of time before and after growth. Fruit flies have three
• The puparium (the hard outer shell of the bug) from the third instar develops and becomes hard and dark.
The puparium is where metamorphosis takes place. Right before the adult fly comes out of the puparium, it darkens.
• About 24 hours before the adult fly emerges, you can see folded wings and eyes if you look closely
o Once metamorphosis is complete, the adult fly pushes its way out our the front end of the piparium.
o First the fruit fly is light with a long abdomen with folded wings, but after a few hours the fly turns darker, the abdomen gets rounder, and the fly speads out it’s wings
• After about 48 hours after the fly comes out of the puparium, females can begin to lay eggs.
o
Tuesday
I posted my application, and I feel good about it. Now I need to start my research paper, but I'm worried about how hard it will be.
Tuesday, January 12, 2010
Monday
I am almost completely done with my application form, all I need to do is change my procedure to make sure that I don't burn all my fruit flies.
Sunday, January 10, 2010
Sunday
I still can't figure out how i am supposed to write about how my two variables relate, but other then that I am done with my application. I emailed ms. weissmen a couple times.
Saturday, January 9, 2010
Saturday
I began to work on my application form, but I am very confused on what to write, I've been asking around, but no else seems to know either. I posted to Form on moodle, and Finally know what my experimental question is, what my hypothesis is, and what my variables are.
I still wish i knew what to write for my application though.
Friday, January 8, 2010
I KNOW MY TOPIC!
Today I went to go see Mrs. Weissmen to make sure I knew what I was doing, and what my topic was. I do now!!! It is the the effect f smoke on the development of fruit flies
Thursday, January 7, 2010
Wednesday, January 6, 2010
Question Template
how does X affect Y?
at what point does X affect Y?
What are the implications of X to Y?
At what point does X not affect Y?
Why does X affect Y?
X= independent
y= dependent
I saw Mrs. Weissman today, and I learned that i just need to research more, especially car exhaust and and cigarette smoke. I need to learn about the DEVELOPMENT OF FRUIT FLIES!!!!!!
at what point does X affect Y?
What are the implications of X to Y?
At what point does X not affect Y?
Why does X affect Y?
X= independent
y= dependent
I saw Mrs. Weissman today, and I learned that i just need to research more, especially car exhaust and and cigarette smoke. I need to learn about the DEVELOPMENT OF FRUIT FLIES!!!!!!
Tuesday, January 5, 2010
list of factors
Abiotic factors:
water, or drought and lack of wawter
temperture extremes
weather
light
fire
gases
rock
snow
land slide
natural disasters: flood, earth quakes
elevation
climate
lack of space
lack of shelter
salinity
UV rays
Sun light
acid rain
humidity
airpressure
air pollution
soil
water polution
radiation
electricity magnetism
electromagnetism
Biotic factor:
food, or lack of food
disease
predation
old age
ingury
water, or drought and lack of wawter
temperture extremes
weather
light
fire
gases
rock
snow
land slide
natural disasters: flood, earth quakes
elevation
climate
lack of space
lack of shelter
salinity
UV rays
Sun light
acid rain
humidity
airpressure
air pollution
soil
water polution
radiation
electricity magnetism
electromagnetism
Biotic factor:
food, or lack of food
disease
predation
old age
ingury
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