Lab report

Today i gave mrs. weissman my new graphs, and i gave mrs. weissman my data analysis, so she could give me pointers. i know most of it is wrong, but i felt like i had to write something over break.

this is all of my lab report i have so far:
Lab report:

Experimental question: “How does different levels smoke affect Drosophila melanogaster development?”

Hypothesis: “ if Drosophila melanogaster are subjected to varying levels of smoke, then the Drosophila melanogaster’s development will be affected.”


Materials:
-1 Graduated cylinder
- 1 pair of goggles
- Painters tape
- 4 73 mL plastic vials
- 1 black sharpie
- 1 plastic spoon
- 1 sheet of paper towel
- 1 1 oz bottle of blue food coloring
- 4 foam plugs
- 6 tea candles (optional: 6 more if the others run out)
- 1 metal box
- 1 ice pick
- 1 microscope
- 135 cc syringe
- 1 paintbrush
- 1 73 mL vial filled with adult fruit flies (ordered from Connecticut Valley Biological Supply)
- 1 magnifying glass
- 1 medium sized box that with not melt
- 1 lighter
- 1 petrie dish
- 1 microscope
- 1 box of matches
- 1 petrir dish lid
- 1 box of Fly Nap
- metal scooper?


Procedure:
1. Take the ice pick, and poke a hole big enough for the syringe tip to go through. Put one on the top and on the side of the metal box.
2. Put a piece of tape over both holes.
3. Take a graduated cylinder and spoon in fruit fly media. Measure out 15 mL.
4. Pour the 15 mL of fruit fly media into a clean plastic vial.
5. Repeat steps 1-4 three times.
6. Take the same graduated cylinder and fill it with 14 mL of water.
7. Take the blue food coloring and drop in 1 mL.
8. Wait for the food coloring and the water to mix, making the liquid a very dark blue.
9. Pour the blue mixture into the same vial with the fruit fly media.
10. Repeat steps 6-8 three times, pouring the mixture in the other vials with the fruit fly media.
11. Add about 3-5 grains of yeast on top of the mixture, which has now become Drosophila medium.
12. Take the painter tape, and put a strip of tape on the side of each vial.
13. Label one “control”, one “small amount of smoke”, one “medium amount of smoke”, one “large amount of smoke”.
14. Wait for the fruit flies to arrive in the mail, and when they do wait for many larva to hatch.
15. Once there are enough larva, get out Fly Nap
16. Open the box and take out the 4 black q-tips inside.
17. Unscrew the cap of the bottle of fly nap and take it off.
18. Take one of the black q-tips and dip it in the Fly Nap, and then pull it out so it is covered in Fly Nap.
19. put the same q-tip into the vial of fruit flys, pushing it donw the side of the vial past the foam plug so no flies escape.
20. Wait 4 minutes for the flies to fall asleep.
21. Once the flies are asleep, take out the foam plug, and nock most of the sleping flies into a petrie dish.
22. Using a metal scooper, scoop the fly larva (along with the medium it’s in) out.
23. Place the fruit larva in one of the empty vials.
24. Repeat steps 15-23 three times.
25. Take the metal box, and take the lid off of it.
26. Light a match and light all the candles in the metal box.
27. Blow them out fast, and slam the lid on top.
28. Wait 10 minutes for the smoke and the air inside to mingle.
29. Take the tape off the top hole of the box and quickly stick the syringe in.
30. Pull out 11 cc of smoke using the syringe.
31. Take the syringe and put the tape back on before any smoke escapes.
32. stick the tip in the side of one of the vials, not taking the plug out
33. Push in the 11 cc of smoke, and pull the tip back out.
34. Repeat steps 25-33 twice, except instead of drawing out 11 ccs draw out 22 cc one time and 33 cc the next.
35. Leave one vial without any smoke. It is the control.
36. Every other day repeat 25-35.
37. Once the flies have hatched out of the puparium and are adults, use fly nap and repeat steps 15- 20.
38. Take out the foam plug and nock out all the flies from the vial onto the petrie dish.
39. Count the number of flies on the dish.
40. Record the number of flies.
41. Nock the flies back into the vial.
42. Place the foam plug back in.
43. Repeat 37-42 with the “small amount of smoke”, “medium amount of smoke”, and “large amount of smoke”.
44. Once done counting the flies fomr each vial, repeat steps 25-35.
45. After about a week there will be New larva in the vials.
46. Repeat steps 3-23, using red food coloring instead of blue.
47. Continue what you do everyday (steps 37-44), except now do it wit the vials wit the blue fruit fly media and vials wit the red fruit fly media.


Independent variable: Different levels of candle smoke

Dependent variable: Fruit fly development

Observations:
date Control Small amount of smoke Medium amount of smoke Large amount of smoke
2/4/10 They seems to be not a lot of larva in the control They are moving around and eating They are moving around and eating They are moving around and eating
2/9/10 Some larva have hatched, and some have gone into the puparium and are undergoing metemorphis. From what I can see 7 new adlut flies have hatched.
Tgere are many in the puparium, but they are to hard ot count.
There are still some larva left. 6 flies have under gone matamorphis and have emerged as an adult fly. There are still some in the puparium though.
I saw 1 larva moving around, so I assume there is more. So far 1 fly has emerged, the rest are in the puparium. A few of the pupariums have darkens, so I know the adult fly will emerge soon. 1 fruit fly has emerged, and the rest are in the puparium. Once again, a few of the pupariums have darkened so I know flys will emerge soon.
2/16/10 The flies are hoping around a lot!!! The flies are not hoping around are as the control. there are less hatched flies The flies are not as active as the control. there are less hatched flies The flies are hoping not as much as control, and there is almost just as much hatched flies in the vial.
2/17/10 There aren’t many puparium, and the oens that are there are darker so that means they’re about to emerge. The flys are flying around, there are some puparium but they are lgiht The flys are flying around, there are some puparium but they are lgiht The flys are flying around, there are some puparium but they are lgiht

Date Observations
2/4/10 They seems to be not a lot of larva in the control
They are moving around and eating
Many are moving around and eating
Many are moving around and eating
2/9/10 Some larva have hatched, and some have gone into the puparium and are undergoing metemorphis. From what I can see 7 new adlut flies have hatched.
Tgere are many in the puparium, but they are to hard ot count.
There are still some larva left.
6 flies have under gone matamorphis and have emerged as an adult fly. There are still some in the puparium though.
I saw 1 larva moving around, so I assume there is more.
So far 1 fly has emerged, the rest are in the puparium. A few of the pupariums have darkens, so I know the adult fly will emerge soon.
1 fruit fly has emerged, and the rest are in the puparium. Once again, a few of the pupariums have darkened so I know flys will emerge soon.
2/16/10 The flies are hoping around a lot!!!
The flies are not hoping around are as the control. there are less hatched flies
The flies are not as active as the control. there are less hatched flies
The flies are hoping not as much as control, and there is almost just as much hatched flies in the vial.
2/17/10 There aren’t many puparium, and the oens that are there are darker so that means they’re about to emerge.
The flys are flying around, there are some puparium but they are lgiht
The flys are flying around, there are some puparium but they are lgiht
The flys are flying around, there are some puparium but they are lgiht

















Date Amount of adults fruit flies when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of adults fruit flies when subjected to 22 ml of smoke Amount of adults fruit flies when subjected to 33 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 11 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 22 ml of smoke Amount of adults fruit flies of the F1 generation when subjected to 33 ml of smoke
2/22/10 55 10 10 12 6 1 0 0
2/24/10 65 27 8 8 8 9 0 0
3/1/10 20 100 20 61 12 36 2 12
3/3/10 19 101 22 25 3 27 0 12
3/5/10 16 111 6 40 1 4 2 9
3/8/10 12 100 6 41 21 1 4 3
3/10/10 12 105 4 28 71 17 2 1

DATA TABLE 1:
The average amount of adult frit flies when subjected to different amounts of smoke over time





DATA TABLE 2:


The Average Amount of Fruit Fly Pupa When Subjected to Different Amounts of Smoke Over Time
Date Amount of fruit fly pupa when subjected to 0 ml of smoke Amount of fruit fly pupa when subjected to 11 ml of smoke Amount of fruit fly pupa when subjected to 22 ml of smoke Amount of fruit fly pupa when subjected to 33 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 0 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 11 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 22 ml of smoke Amount of fruit fly pupa of the F1 generation when subjected to 33 ml of smoke
3/2/10 76 120 7 13 25 53 4 5
3/3/10 90 138 11 22 30 50 4 6
3/4/10 89 140 8 21 42 41 4 5
3/5/10 96 152 8 18 55 88 4 4
3/8/10 109 155 10 23 85 109 3 6
3/9/10 110 188 9 20 83 107 2 6
3/10/10 95 140 9 25 89 131 4 6

The Average Amount of Fruit Fly Pupa When Subjected to Different Amounts of Smoke

Amount of smoke Average of fruit fliy pupa
0 ml of smoke 95
11 ml of smoke 148
22 ml of smoke 9
33 ml of smoke 20
0 ml of smoke F1 generation 58
11 ml of smoke F1 generation 83
22 ml of smoke F1 generation 4
33 ml of smoke F1 generation 6







The average amount of adult frit flies when subjected to different amounts of smoke

Amount of Smoke Average of adult flies
0 ml of smoke 28
11 ml of smoke 79
22 ml of smoke 11
33 ml of smoke 31
0 ml of smoke F1 generation 17
11 ml of smoke F1 generation 13
22 ml of smoke F1 generation 2
33 ml of smoke F1 generation 5


Graph 1:


Graph 2:


Data analysis:

This experiment focused on the affect of smoke on the development of fruit flies, and if there is an effect, determining how extreme it is. Acknowledging the fact that different amounts of smoke could affect the different rates of development of fruit flies, different amounts of smoke were used in this experiment to determine the nature of their relationship. Two kinds of data were taken: the number of live adult fruit flies on a certain day, and the number of fruit fly pupa on a certain day. The results were recorded as quantitative data, using fly nap as an anesthetizer to quite the flies so they could be counted, and a magnifying glass to clearly see the number of fly pupa.
The number of adult fruit flies when subjected to different amounts of smoke over time was recorded in data table 1, and the average per vial of those adult flies was recorded in data table number 3. There were many noticeable trends in these data tables. For one, the number of adult fruit flies was a great deal higher then the number of fruit flies in any other container, including the control. In both the parent generation and the f1 generation, the highest number of live adult fruit flies was in the vial subjected to small amount of smoke. Another notable trend was the fact that number of adult fruit flies that were subjected to the medium amount of smoke was lower than any other vial, including the number of fruit flies that were subjected to the highest amount of smoke. Once again, this trend occurs both in the parent generation and the f1 generation. The highest number of live adult fruit flies in the medium amount of smoke vial was 22, which is very low compared to all the other vials. The vial that held fruit flies that were subjected to the greatest amount of smoke was supposed to have the least amount of flies in it actually had more flies then the vial that had the medium amount of smoke in it, except for one day when both vials had 8 flies. Looking at data table 3 you can see that over all the vial subjected to the least amount of fruit flies had the most live adult flies, the vial subjected to no smoke had the sound largest number of living adult flies, the vial subjected to the largest amount of smoke had the third greatest amount of live adult fruit flies, and the vial that was subjected to the medium amount of smoke had the least amount of live adult fruit flies.
The number of fruit fly pupa each day was recorded in data table 2, and the average of those pupa was recorded in data table 4. A noticeable trend in both of these data tables is the fact that the highest number of pupa was in the vials that were subjected to the least amount of smoke. This trend resembles that of data tables 1 and 3. Another pattern in these data tables are the facts that the number pupa in the vials subjected to medium amount of smoke was very low, and the number of pupa in the vial subjected to a largest amount of smoke was higher. Once again, this trait resembles that of data table 1 and 3.
In conclusion, all 4 of these data tables resemble each other’s patterns and trends. The largest amount of live fruit flies and the largest amount of pupa for both generations occurred in the vial that was subjected to the smallest amount of smoke. It is important to remember that these numbers were higher then those in the vials with no smoke, which was not something that was expected to, happen. The other main trend was the fact that lowest number of live adult fruit flies and the lowest number of pupa occurred in the vials that were subjected to the medium amount of smoke. Another important thing to remember that these numbers were lower then those in the vials subjected to largest amount of smoke, which is another trend that was not predicted to occur.




Conclusion:

Thursday March 11th, 2010

Today I cleaned up my experiment. It was almost sad, but i as happy to see those flies go. They were DISGUSTING! I put the flies in the freezer, so I guess they'll freeze. I also went to see Mrs. Weissman to ask how I should write my data analysis. I think i got a basic idea, but i'll see what she says to us in class. Today I wrote my intro to my analysis i think. this is what I wrote:

Data analysis:

This experiment focused on the affect of smoke on the development of fruit flies, and if there is an affect, determine how extreme it is. Acknowledging the fact that different amounts of smoke could affect the different rates of development of fruit flies, different amounts of smoke were used in this experiment to determine the nature of their relationship. Two kinds of data were taken: the number of live adult fire flies on a certain day, and the number of fruit fly pupa on a certain day. The results were recorded as quantitative data, using fly nap as a anestisiser to quite the flies so they could be counted, and a magnifying glass to clearly see the number of pupa.


That is what i've written so far. I hope it's right.

Wensday march 10th, 2010

Today i finished my experiment!!!!!!!!!! I'm so happy!!!!! those flies were really disgusting. tomorrow I'm going to c ms. Weissman about how to write a data analysis and a conclusion. also, i need to talk to her about what caused my data, because i have no clue still. i have lots to talk to her about. Today i also took lots of photos on my laptop of my experimental design to include in my lab report. I'm going to ask Nadia tomorrow how to label them on the computer.

Tuesday, March 9th, 2010

Today I made my graphs. surprisingly, it was not hard to do! I'm going to the graphing class anyway at CWP just to be safe, but I've successfully made a graph!!! actually I've made 2 graphs! they are very complicated graphs, but everything about them is right i think!! the only problem is they are very very hard to read, so I'm really going to have to explain it when I present. I can't post graphs though, so I'll email them to myself ot back them up!
I think I will ask ms. Weissman about how to write my data analysis tomorrow.

Monday, March 8th, 2010

Today us my second to last day using fly nap to count the adult flies. I'm very excited. I turned my data table in today, so i got those points, which is good. I'm very worried about one thing though. I have no clue why there are so many bugs in "small amount of smoke". And that goes for both the f1 generation and the f2 generation. smoke can't be actually good for them, can it? I'm very confused ,and I'm worried about what I'll say in my conclusion. I'm also worried about writing my data analysis. i know it's supposed to be long, but i do not have a lot data, so I'm not really sure what i write about. Today i got more data, which is good. I counted the adult flies and he pupa. this is what i got.

PUPA:(in the order of: control, small, medium, large, control f2, small f2, medium f2, large f2)

3/8/10 109 155 10 23 85 109 3 6

ADULTS: (in the order of: control, small, medium, large, control f2, small f2, medium f2, large f2)

3/8/10 12 100 6 41 21 1 4 3

Saturday March 6th

Yesterday I went and talked to ms. Weissman, and decided i need to make new data tables. Now i'm going ot have 2 different data tables, one for number of adutls, and one for number of pupa. this is what i ended up with:

Date Amount of adults fruit flies when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of adults fruit flies when subjected to 22 ml of smoke Amount of adults fruit flies when subjected to 33 ml of smoke
2/22/10 55 10 10 12
2/24/10 65 27 8 8
3/1/10 20 100 20 61
3/3/10 19 101 22 25
3/5/10 16 111 6 40

Date Amount of fruit fly pupa when subjected to 0 ml of smoke Amount of adults fruit flies when subjected to 11 ml of smoke Amount of fruit fly pupa when subjected to 22 ml of smoke Amount of fruit fly pupa when subjected to 33 ml of smoke
3/2/10 76 120 7 13
3/3/10 90 138 11 22
3/4/10 89 140 8 21
3/5/10 96 152 8 18

Date Amount of fruit fly pupa of the F2 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 11 ml of smoke Amount of fruit fly pupa of the F2 generation when subjected to 22 ml of smoke Amount of fruit fly pupa of the F2 generation when subjected to 33 ml of smoke
3/2/10 25 53 4 5
3/3/10 30 50 4 6
3/4/10 42 41 4 5
3/5/10 55 88 4 4

Date Amount of adults fruit flies of the F2 generation when subjected to 0 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 11 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 22 ml of smoke Amount of adults fruit flies of the F2 generation when subjected to 33 ml of smoke
2/22/10 6 1 0 0
2/24/10 8 9 0 0
3/1/10 12 36 2 12
3/3/10 3 27 0 12
3/5/10 1 4 2 9


it's hard t format in this blog. my data makes no sense to me what's so ever, and I need ot go see ms. weissman when i'm done taking it. I'm very worried that i won't be able to explain it in my conclusion, because i have ablutly no clue why the small amount of smoke is so much higher the the control. it can't just be because of the excess amount of fly nap, because that same trand is in the f2 gernation as well. I hope i can figure out what happened.

Thursday, march 4th, 2010

Today I did not count my fruit flies, because i don't want to kill them by anesticising them everyday. I'm kind of worried about my lack of data (4 days counting flies, 2 counting pupa) but I hope I will have enough to graph by Wednesday. this what i got today:

3/4/10 Control Pupa: 89 Control F2 Pupa: 42
Small Pupa: 140 Small f2 Pupa: 41
Medium Pupa: 8 Medium F2 Pupa:4
Large Pupa: 21 Large F2 Pupa: 5

Before my data was all messed up because i wasn't able to add smoke to the fruit flies due to the snow days, but that i've been doing it for a few days finally i think my data is getting back on track, or atleast the pupa data is. yesterday there were 30 pupa in the F2 control vial and now there is 42. the flies are developing fast in the control, and not as fast in any of the others. Maybe i will end up with the results i predicited! Only time will tell.